67309

Kovac′s reagent for indoles

suitable for microbiology

• Sinônimo(s): 4-(Dimethylamino)benzaldehyde solution
• Fórmula empírica (Notação de Hill): C₉H₁₁NO
• Número CAS: 100-10-7
• Peso molecular: 149.19
• Beilstein: 4132845
• Número MDL: MFCD00003381
• Código UNSPSC: 41171621
• ID de substância PubChem: 329760541
• NACRES: NA.85

Propriedades

• Agency: according to ISO 16654:2001
• Nível de qualidade: 200
• linha de produto: BioChemika
• prazo de validade: limited shelf life, expiry date on the label
• composição: 4-(dimethlyamino)benzaldehyde, 50 g/L ; hydrochloric acid, 240 g/L ; isoamylic alcohol, 710 g/L
• técnica(s): microbe id | specific enzyme detection: suitable
• aplicação(ões): agriculture; clinical testing; environmental; food and beverages; microbiology
• temperatura de armazenamento: 2-8°C
• adequação: Escherichia coli; coliforms
• cadeia de caracteres SMILES: [H]C(=O)c1ccc(cc1)N(C)C
• InChI: 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
• chave InChI: BGNGWHSBYQYVRX-UHFFFAOYSA-N

Descrição

Descrição geral

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Aplicação

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.

Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.

Informações de segurança

• Pictogramas: GHS02,GHS05,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H226,H290,H314,H317,H332,H335
• Declarações de precaução: P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classificações de perigo: Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 3 - Flammable liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): 109.4 °F
• Ponto de fulgor (°C): 43 °C
• Equipamento de proteção individual: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter