1.11350

Bactident^™ Kovac′s Indole reagent

Available in a practical dropper bottle, for Escherichia coli

• Sinônimo(s): 4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent
• Código UNSPSC: 41106200
• NACRES: NA.84

Propriedades

• Nível de qualidade: 100
• descrição: Bactident™ E. coli 1.13303
• forma: liquid (ready-to-use)
• prazo de validade: 210 days
• Características: ready-to-use
• composição: (n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)
• embalagem: pkg of (1 x 30 ml, Plastic bottle)
• técnica(s): microbe id | specific enzyme detection: suitable
• pH: <1 (20 °C in H₂O)
• temperatura de transição: flash point 36 °C
• densidade: 0.92 g/cm³ at 20 °C
• aplicação(ões): environmental; food and beverages
• temperatura de armazenamento: 2-8°C
• adequação: Enterobacter spp.; Escherichia coli

Descrição

Descrição geral

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

Aplicação

Bactident^™ Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

Ligação

Replaces: 1.11350.0007

Nota de análise

Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.

Informações legais

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS02,GHS05,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H226 - H290 - H302 - H314 - H317 - H335 - H336
• Declarações de precaução: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Classificações de perigo: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1
• Código de classe de armazenamento: 3 - Flammable liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): 96.8 °F
• Ponto de fulgor (°C): 36 °C