FORMD-RO

Formate Dehydrogenase

from Candida boidinii

• Sinônimo(s): FDH, Formate:NAD oxidoreductase
• Número da licença da enzima: 1.2.1.2 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propriedades

• fonte biológica: yeast (Candida boidini)
• Nível de qualidade: 100
• recombinante: expressed in E. coli
• forma: lyophilized
• atividade específica: ~0.4 units/mg protein (At 25 °C with formate as the substrate.)
• peso molecular: 74 kDa
• embalagem: pkg of 250 U (10837016001); pkg of 80 U (10244678001)
• fabricante/nome comercial: Roche
• concentração: ≥10-20 % (w/w)
• técnica(s): activity assay: suitable
• cor: off-white
• pH ideal: 7.5-8.5
• solubilidade: water: soluble 10 g/L
• adequação: suitable for gel electrophoresis
• nº de adesão UniProt: O13437
• aplicação(ões): life science and biopharma
• atividade externa: ADH <0.0500%; LDH <0.0500%; MDH <0.1000%
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Formate:NAD⁺ oxidoreductase
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.

Especificidade

Substrate Specificity and K_m:
Formate dehydrogenase reacts only with formate (K_m = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (K_m 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.

Aplicação

Most widely used in cofactor recycling systems for NADH.

Qualidade

Contaminants: <0.005% LDH and ADH each, <0.1% MDH

Definição da unidade

One unit (U) formate dehydrogenase will oxidize 1 μmol of formic acid to CO₂ in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized.

Armazenamento e estabilidade

Store dry

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash