10108014001

Papain

from Carica papaya

• Sinônimo(s): cystein protease, papain
• Número da licença da enzima: 3.4.22.2 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propriedades

• fonte biológica: papaya
• Nível de qualidade: 100
• Formulário: suspension
• atividade específica: 30 U/mg; ~30 units/mg protein (At 25 °C with BAEE as the substrate.)
• peso molecular: 23 kDa
• embalagem: pkg of 10 mL (100 mg)
• fabricante/nome comercial: Roche
• concentração: 10-100 mg/mL
• pH ideal: 6.0-7.0
• Condições de expedição: wet ice

Descrição

Descrição geral

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Aplicação

Papain has been used in the digestion of various brain cells.

Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

Ações bioquímicas/fisiológicas

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

Características e benefícios

Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals

forma física

Suspension, crystalline, nonsterile

Nota de preparo

Working concentration: 0.05 to 0.5 mg/ml

Armazenamento e estabilidade

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash