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SCM132

Sigma-Aldrich

PluriSTEM-XF® Human ES/iPS Cell Medium

PluriSTEM-XF Human ES/iPS Medium is a complete xeno-free medium formulation for the feeder-free culture of human ES and iPS cells.

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About This Item

Código UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Nível de qualidade

forma

liquid

fabricante/nome comercial

PluriSTEM®

técnica(s)

cell culture | embryo: suitable
cell culture | stem cell: suitable

Condições de expedição

dry ice

Categorias relacionadas

Descrição geral

PluriSTEM-XF Human ES/iPS Medium is a complete xeno-free medium formulation for the feeder-free culture of human ES and iPS cells. Pluripotent cells maintained in thissmall molecule based medium exhibit high cell health, express high levels of pluripotency markers (NANOG, OCT3/4, SOX-2, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), retain differentiation potential and possess a normal karyotype. PluriSTEM-XF Human ES/iPS Medium is provided as a stand-alone 500 mL bottle that is ready-to-use and does not require additional supplementation.

For a complete xeno-free culture system, PluriSTEM-XF Human ES/iPS Medium should be used with PluriSTEM-XF Recombinant Vitronectin (Cat. No. CC130).

Each lot of PluriSTEM-XF Human ES/iPS Medium is rigorously quality control tested for the ability to sustain undifferentiated human ES/iPS cells for 3 passages.

Note: Before starting PluriSTEM-XF, users are recommended to read the manual carefully and completely. Colony morphology and growth rates may differ from other serum-free, feeder-free media culture conditions.

Aplicação

Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Qualidade

Each lot of PluriSTEM® Human ES/iPS Medium is rigorously quality control tested for the ability to sustain undifferentiated human ES cells for greater than 3 passages.

Armazenamento e estabilidade

PluriSTEM-XF Human ES/iPS Medium is provided frozen and can be stored at -20°C until the expiration date on the product label.

Before use, thaw PluriSTEM-XF medium at room temperature (15 – 25°C) or overnight at 2 – 8°C. Do not thaw at 37°C. Upon thawing, PluriSTEM-XF medium may be aseptically dispensed into working aliquots and stored at -20°C until the expiration date as indicated on the label. Thawed aliquots may be stored at 2 – 8°C for up to 2 weeks. Do not refreeze aliquots after thawing. Before use, warm working aliquots to room temperature. Do not warm the medium in a 37°C water bath.

Informações legais

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Artigos

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmental pathways and help regulate mesoderm and ectoderm patterning in the early embryonic development.

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

Protocolos

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

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