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SCM075

Sigma-Aldrich

Inibidor de ROCK (Y-27632)

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

Sinônimo(s):

Y-27632

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About This Item

Código UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Nível de qualidade

100

forma

liquid

peso molecular

320.26 g/mol

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

dry ice

Descrição geral

O inibidor de ROCK (Y-27632) é um inibidor competitivo com ATP e seletivo de quinases proteicas associadas a Rho (ROCK), incluindo a p160Rock (Ki=140nM) e a Rock-II. Também age como potente inibidor da sensibilização a Ca2+ induzida por agonista da fosforilação de miosina e contração de músculo liso. Um tratamento de uma hora com 10uM de Y-27632 bloqueia a apoptose de células-tronco embrionárias humanas (CTEh) cultivadas dissociadas, aumentando a sobrevivência e a eficiência de clonagem das CTEh sem afetar sua pluripotência1. Recentemente, alguns relatos demonstraram que a aplicação de Y-27632 e Accutase (N.º do cat. SCR005), uma mistura formulada de enzimas digestivas, aumenta a taxa de sobrevivência pós-descongelamento de CTEh individuais usando métodos convencionais de congelamento lento e descongelamento rápido

Armazenamento e estabilidade

Antes da reconstituição: Manter a -20 ºC, ao abrigo da luz por 2 anos. Após a reconstituição: 1 ano a -20 ºC, ao abrigo da luz

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Stem Cell Qualified ECM Gel Stem cell qualified basement membrane extract (BME) for human ES and iPS cell culture.

Sigma-Aldrich

CC131

Stem Cell Qualified ECM Gel

A 83-01 ≥98% (HPLC)

Sigma-Aldrich

SML0788

A 83-01

Katarzyna Pańczyk et al.
MedChemComm, 9(11), 1933-1948 (2018-12-21)
A series of 17 new phenoxyacetamides has been prepared via multistep chemical synthesis as a continuation of the research carried out by our group on di- and tri-substituted phenoxyalkyl and phenoxyacetyl derivatives of amines. The obtained compounds vary in an
Maike Frye et al.
eLife, 9 (2020-09-09)
Endothelial integrity is vital for homeostasis and adjusted to tissue demands. Although fluid uptake by lymphatic capillaries is a critical attribute of the lymphatic vasculature, the barrier function of collecting lymphatic vessels is also important by ensuring efficient fluid drainage
Xin Geng et al.
JCI insight, 5(14) (2020-06-17)
During the growth of lymphatic vessels (lymphangiogenesis), lymphatic endothelial cells (LECs) at the growing front sprout by forming filopodia. Those tip cells are not exposed to circulating lymph, as they are not lumenized. In contrast, LECs that trail the growing
Anushree C Gulvady et al.
Molecular biology of the cell, 30(11), 1298-1313 (2019-03-21)
Fibroblasts transformed by the proto-oncogene Src form individual invadopodia that can spontaneously self-organize into large matrix-degrading superstructures called rosettes. However, the mechanisms by which the invadopodia can spatiotemporally reorganize their architecture is not well understood. Here, we show that Hic-5
Xin Lin et al.
EBioMedicine, 69, 103463-103463 (2021-07-06)
Family with Sequence Similarity 13, Member A (FAM13A) gene has been consistently associated with COPD by Genome-wide association studies (GWAS). Our previous study demonstrated that FAM13A was mainly expressed in the lung epithelial progenitors including Club cells and alveolar type
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