QIA33

FragEL DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme

• Sinônimo(s): TUNEL Assay
• Código UNSPSC: 41116133
• NACRES: NA.51

Propriedades

• uso: sufficient for 50 tests
• Nível de qualidade: 100
• reatividade da espécie (prevista por homologia): all
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• técnica(s): microbe id | specific enzyme detection: suitable
• entrada: sample type paraffin section(s); sample type frozen section(s); sample type fixed-cell preparation(s)
• método de detecção: colorimetric
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Non-isotopic kit for the detection of apoptosis at the individual cell level. Apoptotic morphology is easily detected. This kit allows the recognition of apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, or in cell preparations fixed on slide by Fragment End Labeling (FragEL) of DNA. Terminal deoxynucleotidyl transferase (TdT) binds to exposed ends of DNA fragments generated in response to apoptotic signals and catalyzes the template-dependent addition of biotin-labeled and unlabeled deoxynucleotides. Biotinylated nucleotides are detected using streptavidin-horseradish peroxidase (HRP) conjugate. Diaminobenzidine reacts with the labeled sample to generate an insoluble colored product at the site of DNA fragmentation. Counterstaining with methyl green aids in the morphological evaluation and characterization of normal and apoptotic cells.

Componentes

Counterstain, Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Stop Buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H₂O₂/Urea Tablets, Methyl Green Counterstain, and a user protocol.

Advertência

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Princípio

The Calbiochem TdT-FragEL DNA Fragmentation Detection Kit is a non-isotopic system for the labeling of DNA breaks in apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, and in cell preparations fixed on slides.

Nota de preparo

1. Generation of Negative Control SamplesA negative control of your specific sample can be generated by substituting dH₂O for the TdT in the reaction mixture or by keeping the specimen in 1X reaction buffer (with a cover slip) during the labeling step. Perform all other steps as described. This controls for endogenous peroxidases and non-specific conjugate binding. A non-apoptotic control is also critical since cells and tissue begin to undergo apoptosis from the very beginning of the excision, fixation, and processing steps. A delay in fixation or routine mechanical manipulation may result in the unwanted breakage of DNA that could be misinterpreted as apoptosis.2. Generation of Positive Control Samples: A positive control of your specific tissue sample can be generated by covering the entire specimen with 1 µg/µl DNase I in 1X TBS/1 mM MgSO₄ following proteinase K treatment. Incubate at room temperature for 20 min. Perform all other steps as described. This will fragment DNA in normal cells. Cytoplasmic as well as nuclear DAB staining of DNase treated cells may be observed.

Armazenamento e estabilidade

Upon arrival store the entire contents of the kit at -20°C in a non-frostfree freezer. Following initial thaw store the Stop Buffer and Methyl Green Counterstain at room temperature and the Blocking Buffer at 4°C. Refreezing of these components, however, does not appear to affect their performance.

Outras notas

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

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Martin, S.J., et al. 1990. J. Immunology145, 1859.
Wyllie, A. H. 1980. Nature284, 555.
Kerr, J. F. R., et al. 1972. Br. J. Cancer26, 239.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS03,GHS08,GHS05,GHS07,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H272,H302 + H332,H315,H317,H318,H341,H350,H373,H411
• Declarações de precaução: P210 - P273 - P280 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Classificações de perigo: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Ox. Liq. 3 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Inhalation
• Órgãos-alvo: Respiratory Tract
• Código de classe de armazenamento: 5.1B - Oxidizing hazardous materials