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Key Documents

APOBRDU

Sigma-Aldrich

Flow Cytometry Kit for Apoptosis

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.32

uso

sufficient for 60 cell suspensions

embalagem

pkg of 1 kit

técnica(s)

flow cytometry: suitable

aplicação(ões)

cell analysis
detection

método de detecção

fluorometric

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Categorias relacionadas

Aplicação

The greater incorporation of Br-dUTP results in a stronger signal by flow cytometry when detected using a fluorescein-labeled anti-BrdU antibody.Propidium iodide/RNase A solution is included in the kit to counterstain the total DNA. APOBRDU has been used in flow cytometry assay in human non–small cell lung cancer. APOBRDU has been used in cell cycle and apoptosis assay in HeLa cells, and in tunel assay human neuroblastoma cell lines.

Características e benefícios

Greater incorporation of Br-dUTP, resulting in improved detection than found by using biotin- or digoxigenin-conjugated dUTP or by using fluorochrome (fluorescein or BODIPY)-conjugated deoxynucleotides.

Embalagem

The kit is shipped in two parts, both wet ice. Upon receipt, store APO-PART1 at −20 °C and APO-PART2 at 2-4 °C

Princípio

Br-dUTP is incorporated more readily into the DNA fragments than deoxyuridine triphosphate labeled with larger dyes such as FITC, biotin or digoxigenin. One of the biological characteristics that defines apoptosis is the degradation of genomic DNA into fragments of 180-200 bp, commonly called "DNA laddering". The fragmentation creates a large number of 3′-hydroxyl sites at the DNA breaks. This property is used in the APO-BRDU kit to identify apoptotic cells by labeling the 3′-hydroxyl sites with bromodeoxyuridine triphosphate (Br-dUTP). Br-dUTP is enzymatically attached to the 3-hydroxyl sites of double- or single-stranded DNA by terminal transferase (TdT). Non-apoptotic cells do not incorporate Br-dUTP due to the lack of available 3-hydroxyl sites.

Somente componentes do kit

Nº do produto
Descrição
  • Br-dUTP 480 μL
  • Fluorescein PRB-1 antibody 300 μL
  • Negative control cells 5 mL
  • PI/RNase staining buffer 30 mL
  • Positive control cells 5 mL
  • Reaction buffer .6 mL
  • Rinsing buffer 120 mL
  • Terminal deoxynucleotidyl transferase (TdT) 45 μL
  • Wash buffer 120 mL
Ver tudo (9)

Pictogramas

FlameHealth hazard
GHS02,GHS08

Palavra indicadora

Danger

Frases de perigo

H225,H317,H319,H334,H350

Classificações de perigo

Carc. 1B - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

3 - Flammable liquids

Ponto de fulgor (°F)

55.4 °F

Ponto de fulgor (°C)

13 °C

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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