MABT456

Anti-MSN Antibody, clone 2C12

ascites fluid, clone 2C12, from mouse

• Sinônimo(s): Moesin, Membrane-organizing extension spike protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: ascites fluid
• tipo de produto de anticorpo: primary antibodies
• clone: 2C12, monoclonal
• reatividade de espécies: human
• técnica(s): flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG1
• nº de adesão NCBI: NP_002435
• nº de adesão UniProt: P26038
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... MSN(4478)

Descrição

Descrição geral

Moesin (membrane-organizing extension spike protein) is the protein encoded by the MSN gene. The MSN encoded protein moesin is a member of the ERM (ezrin, moesin, radixin) family of proteins that regulate linkage between the plasma membrane and the actin cytoskeleton. Thus, the MSN encoded protein is a peripheral membrane protein that is keenly involved in the signaling and structural connections in cytoskeletal plasma membrane structures like microvilli. Moesin in its active, phosphorylated state strongly binds actin and helps anchor it to the plasma membrane. The MSN encoded Moesin is localized to filopodia and other membranous extensions of various cell types and thus plays a role in cell-cell recognition signaling, cell movement, and cell transport. In its inactive, non-phosphorylated state, Moesin binds itself in a head-tail fashion and is incapable of binding actin or other cytoskeletal proteins. The kinase ROCK2 is central to the opening up of moesin and allowing its interaction with actin and the plasma membrane. EMD-Millipore’s Anti-MSN, clone 2C12 has been tested in western blots against purified recombinant protein as well as multiple human cancer cell lysates. The clone has also been tested successfully in paraffin embedded immunohistochemistry on human colon tissue and by flow cytometry on human Jurkat cells and ELISA using purified recombinant antigen.

Imunogênio

Purified protein fragment of human MSN expressed in E. coli.

Aplicação

Detect Moesin using this mouse monoclonal antibody, Anti-MSN Antibody, clone 2C12 validated for use in western blotting, IHC & Flow Cytometry.

Immunohistochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected MSN in human tonsil and colon tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MSN in Jurkat cells.

Optimal working dilutions must be determined by end user.

Qualidade

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MSN in 10 µg of Jurkat cell lysate.

Descrição-alvo

~68 kDa observed. Uncharacterized bands may appear in some lysate(s).

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H360D,H412
• Declarações de precaução: P202 - P273 - P280 - P308 + P313 - P405 - P501
• Classificações de perigo: Aquatic Chronic 3 - Repr. 1A
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable