MABN845

Anti-Neurophysin 2/NP-AVP Antibody, clone PS 41

clone PS 41, from mouse

• Sinônimo(s): Vasopressin-neurophysin 2-copeptin, AVP-NPII, Arg-vasopressin, Arginine-vasopressin, Neurophysin 2, Neurophysin-I, Copeptin, Neurophysin 2/NP-AVP
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: PS 41, monoclonal
• reatividade de espécies: rat
• técnica(s): ELISA: suitable; electron microscopy: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; radioimmunoassay: suitable; western blot: suitable
• Isotipo: IgG2bκ
• nº de adesão NCBI: NP_058688
• nº de adesão UniProt: P01186
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: rat ... Avp(24221)

Descrição

Descrição geral

The neurophysin 1 and 2 (NPI and NPII) are carriers of peptide hormone oxytocin (OT) and vasopressin (AVP), respectively. Each NP and its associated hormone peptide are synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or AVP-NPII (UniProt P01186) encoded by the Avp gene in rat species. Post-translational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI) or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII). OT is a potent hormone and neurotransmitter secreted within the pituitary gland. OT exerts its biological activity through its receptor OXTR (also known as OTR). OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders. AVP functions primarily as an anti-diuretic and regulates the concentration of water, glucose, and salts in blood. AVP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R).

Especificidade

Clone PS 41 reacts with Vasopressin-neurophysin 2-copeptin precursor, neurophysin 2, but not Arg-vasopressin, copeptin, oxytocin, or neurophysin 1.

Imunogênio

Epitope: Neurophysin 2

KLH-conjugated acid soluble extract of rat (Sprague-Dawley) intermediate lobes.

Aplicação

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Neurophysin 2/NP-AVP in rat pituitary tissue.
Immunohistochemistry Analysis: A representative lot detected Neurophysin 2/NP-AVP immunoreactivity in the hypothalamus of developing rats as early as embryonic day (E16) using coronal vibratome sections. (Witnall, M.H., et al.(1985). J Neurosci. 5(1):98-109).
Radioimmunoassay Analysis: Target reactivity of a representative lot was confirmed by RIA using plates coated with purified rat Neurophysin/NP and 125I-labelled secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Electron Microscopy Analysis: A representative lot immunostained granulated neurosecretory vesicles (NSVs) in all axons, Herring bodies, and terminals by EM using rat posterior pituitary LR White sections (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Immunoprecipitation Analysis: A representative lot immunoprecipitated radiolabelled neurophysin 2, but not neurophysin 1 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
ELISA Analysis: A representative lot detected neurophysin 2 immunoreactivity in rat neurophysin preparations by non-sandwich ELISA using either 125I-labelled or HRP-conjugated secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This Anti-Neurophysin 2/NP-AVP Antibody, clone PS 41 is validated for use in Western Blotting and Immunohistochemistry, Radioimmunoassay, Electron Microscopy, Immunoprecipitation and ELISA for the detection of Neurophysin 2/NP-AVP.

Qualidade

Evaluated by Western Blotting in rat pituitary tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Neurophysin 2/NP-AVP in 10 µg of rat pituitary tissue lysate.

Descrição-alvo

~15 kDa observed

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable