MABE1121

Anti-Ago1 Antibody, clone 4B8

clone 4B8, from rat

• Sinônimo(s): Protein argonaute-1, Argonaute1, hAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding protein Q99, Ago1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propriedades

• fonte biológica: rat
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 4B8, monoclonal
• reatividade de espécies: human
• técnica(s): western blot: suitable
• Isotipo: IgG2aκ
• nº de adesão NCBI: NP_036331.1
• nº de adesão UniProt: Q9UL18
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... AGO1(26523)

Descrição

Descrição geral

Protein argonaute-1 (UniProt Q9UL18; also known as Argonaute1, Argonaute RISC catalytic component 1, eIF-2C 1, eIF2C 1, Eukaryotic translation initiation factor 2C 1, hAgo1, Putative RNA-binding protein Q99) is encoded by the AGO1 (also known as EIF2C, EIF2C1) gene (Gene ID 26523) in human. Ago1 belongs to the family of argonaute proteins involved in gene expression by regulating gene transcription and RNA splicing in the nucleus, as well as by regulating posttranscriptional gene silencing mediated by siRNAs and microRNAs in the cytosol. Ago1 is shown to interact with chromatin modifiers, splicing factors, and RNA polymerase II. Ago1 and the related Ago2 are also shown to target Hepatitis C Virus (HCV) RNÁ 5′-untranslated region (5′-UTR) in an miR-122-dependent manner, resulting in viral RNA stability and translation stimulation.

Especificidade

Clone 4B8 sepcifically immunoprecipitated FLAG-tagged Ago1,but not FLAG-tagged Ago2, Ago3, or Ago4 (Ender, C., et al. (2008). Mol. Cell. 32(4):519-528).

Imunogênio

GST-tagged recombinant human Ago1.

Aplicação

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Ago1 in 10 µg of K562 cell lysate.
Western Blotting Analysis: A representative lot detected Ago1-containing messenager ribonucleoprotein (mRNP) complexes primarily in the low density fractions of sucrose gradient-fractionated HEK293 lysate (Höck, J., et al. (2007). EMBO Rep. 8(11):1052-1060).
Immunoprecipitation Analysis: Representative lots immunoprecipitated FLAG-tagged Ago1, but not FLAG-tagged Ago2, Ago3, or Ago4 (Ender, C., et al. (2008). Mol. Cell. 32(4):519-528; Beitzinger, M., et al. (2007). RNA Biol. 4(2):76-84).
RNA Binding Protein Immunoprecipitation (RIP): Representative lots co-immunoprecipitated Ago1-associated RNAs, including mRNAs, miRNAs, and snoRNAs from human cell lysates (Li, Z., et al. (2009). J. Virol. 83(24):12751-12758; Ender, C., et al. (2008). Mol. Cell. 32(4):519-528; Beitzinger, M., et al. (2007). RNA Biol. 4(2):76-84).

Qualidade

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Ago1 in 10 µg of HeLa cell lysate.

Descrição-alvo

~97 kDa observed

forma física

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable