Pular para o conteúdo
Merck
Todas as fotos(4)

Documentos Principais

MABC953

Sigma-Aldrich

Anti-Prohibitin-2/PHB2 Antibody, clone 7F8E3

clone 7F8E3, from rat

Sinônimo(s):

Prohibitin-2, B-cell receptor-associated protein BAP37, D-prohibitin, Repressor of estrogen receptor activity, Prohibitin-2/PHB2

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rat

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

7F8E3, monoclonal

reatividade de espécies

rat, monkey, human, mouse

técnica(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

Isotipo

IgG2aκ

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... PHB2(11331)

Descrição geral

Prohibitin-2 (UniProt Q99623; also known as B-cell associated protein, B-cell receptor-associated protein BAP37, BCAP37, D-prohibitin, PHB2, Repressor of estrogen receptor activity) is encoded by the PHB2 (also known as BAP, REA) gene (Gene ID 11331) in human. Prohibitins are assembled into a ring-like PHB complex with 16-20 alternating Phb1 and Phb2 subunits in the inner mitochondrial membrane, where they function as chaperone for respiration chain proteins or as important structural scaffold for optimal mitochondrial morphology and function. Prohibitins are also reported to modulate transcriptions by interacting with various transcription factors, including nuclear receptors. Upregulated expression of prohibitins, septin 9, neurabin 1, and other cytoskeletal and functional proteins is found at the glutathione S-transferase placental-form-positive (GST-P+) hepatocellular carcinoma (HCC) foci during initiation and/or promotion stages of rat hepatocarcinogenesis, implicating an involvement of prohibitins in the neoplastic transformation of rat liver preneoplastic lesions.

Especificidade

Expected to react with both spliced isoforms.

Imunogênio

Epitope: Near C-terminus
KLH-conjugated linear peptide corresponding to human Prohibitin-2/PHB2.

Aplicação

Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-Prohibitin-2/PHB2 Antibody, clone 7F8E3 is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin) for the detection of Prohibitin-2/PHB2.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Prohibitin-2/PHB2 in 10 µg of COS-7 cell lysate.
Immunocytochemistry Analysis: 4 µg/mL from a representative lot detected Prohibitin-2/PHB2 in A431 and HUVEC cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Prohibitin-2/PHB2 in human breast and mouse liver tissue.
Immunohistochemistry Analysis: A representative lot detected upregulated Prohibitin-2 immunoreactivity at the glutathione S-transferase placental-form–positive (GST-P+) hepatocellular carcinoma (HCC) foci in frozen liver sections from rats subjected to hepatocarcinogenesis induction by N-nitrosodiethylamine (DEN) and Phenobarbital (PB) treatment (Kakehashi, A., et al. (2011). Toxicol Sci. 119(1):61-72).

Qualidade

Evaluated by Western Blotting in rat C6 glioma cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Prohibitin-2/PHB2 in 10 µg of rat C6 glioma cell lysate.

Descrição-alvo

~34 kDa observed

forma física

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Não está encontrando o produto certo?  

Experimente o nosso Ferramenta de seleção de produtos.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Anna Kakehashi et al.
Toxicological sciences : an official journal of the Society of Toxicology, 119(1), 61-72 (2010-10-12)
In the present study, protein lysates from microdissected glutathione S-transferase placental-form-positive (GST-P(+)) foci and hepatocellular carcinomas from livers of rats treated with N-diethylnitrosamine followed by phenobarbital at doses of 0 and 500 ppm in the diet for 10 and 33
Sunkyu Choi et al.
Scientific reports, 8(1), 5858-5858 (2018-04-13)
Cancer metastasis causes approximately 90% of all cancer-related death and independent of the advancement of cancer therapy, a majority of late stage patients suffers from metastatic cancer. Metastasis implies cancer cell migration and invasion throughout the body. Migration requires the

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica