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Merck
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Documentos Principais

MAB3806-C

Sigma-Aldrich

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Ascites Free

clone 10H11.E12, from mouse

Sinônimo(s):

Serine-protein kinase ATM, Ser1981 phosphorylated, Ataxia telangiectasia mutated, Ser1981 phosphorylated, A-T mutated, Ser1981 phosphorylated

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

10H11.E12, monoclonal

reatividade de espécies

mouse, human

técnica(s)

ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1κ

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

phosphorylation (pSer1981)

Informações sobre genes

human ... ATM(472)

Descrição geral

Serine-protein kinase ATM (EC 2.7.11.1; UniProt Q13315; also known as A-T mutated, Ataxia telangiectasia mutated) is encoded by the ATM (also known as AT) gene (Gene ID 472) in human. Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). Known ATM substrates include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1. The S/TQ sequence constitutes the essential substrates phosphorylation site motif. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate phosphorylation, while positively charged residues surrounding the S/TQ are negative determinants. The complex phenotypes of cells derived from patients with AT suggests that ATM has additional cellular substrates. ATM is present as an inactive homodimer or multimer prior to activation. DNA double-stranded breaks induced by ionizing radiation (IR) cause rapid ATM autophosphorylation at Ser1981 in human or the Ser1987 equivalent in mouse.

Especificidade

Reacts with ATM Kinase phosphorylated at serine 1981. Clone 10H11.E12 is covered by US patent No. 6,916,627 and 7,108,992.

Imunogênio

Epitope: pSer1981.
Synthetic peptide corresponding to a.a. 1974-1988 of human ATM with phosphorylated Ser1981 (SLAFEEG[pS]QSTTISS).

Aplicação

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Ascites Free is an antibody against phospho-ATM for use in Immunocytochemistry, Immunoprecipitation, ELISA, Western Blotting.
Immunoprecipitation Analysis: A representative lot detected Ser1981-phosphorylated ATM interaction with endogenous EphA5 by co-immunoprecipitation using chromatin-enriched protein fractions of irradiated NCI-H460 human lung cancer cells (Staquicini, F.I., et al. (2015). J. Biol. Chem. 290(12):7345-7359).
ELISA Analysis: a representative lot was employed as the capture antibody for the detection of interaction between EphA5 and Ser1981-phosphorylated ATM. ATM pSer1981 captured from NCI-H460 human lung cancer cell extracts interacted with recombinant EphA5 cytoplasmic domain, but not with EphA5 extracellular domain or kinase domain (Staquicini, F.I., et al. (2015). J. Biol. Chem. 290(12):7345-7359).
Immunocytochemistry Analysis: A representative lot detected radiation-/IR-induced upregulation of nuclear ATM pSer1981 foci by fluorescent immunocytochemistry staining of methanol-fixed NCI-H460 human lung cancer cells (Staquicini, F.I., et al. (2015). J. Biol. Chem. 290(12):7345-7359).
Immunocytochemistry Analysis: A representative lot detected radiation-/IR-induced ATM Ser1981 phosphorylation by fluorescent immunocytochemistry staining of 1.2% formaldehyde-fixed, 0.1% Triton X-100-permeabilized HT29 cells (Bardelle, C., and Boros, J. (2012). J. Biomol. Screen. 17(7):912-920).
Immunocytochemistry Analysis: A representative lot detected ionizing radiation-/IR-induced ATM Ser1981 phosphorylation by fluorescent immunocytochemistry staining of methanol/acetone-fixed primary human foreskin fibroblasts (HFFs) (Bakkenist, C.J., et al. (2004). Cancer Res. 64(11):3748-3752).
Western Blotting Analysis: A representative lot detected epirubicin-induced ATM Ser1981 (Ser1987 in mouse) phosphorylation in mouse embryonic fibroblasts (MEFs) and human MCF-7 cells (Khongkow, P., et al. (2014). Oncogene. 33(32): 4144-4155).
Western Blotting Analysis: A representative lot detected camptothethin-induced ATM Ser1987 (Ser1981 in human) phosphorylation in primary mouse cortical neurons (Brochier, C., et al. (2013). J. Neurosci. 33(20): 8621-8632).
Western Blotting Analysis: A representative lot detected radiation-/IR-induced ATM Ser1981 phosphorylation in HT29, HeLa, and HEK293T cells (Bardelle, C., and Boros, J. (2012). J. Biomol. Screen. 17(7):912-920).
Western Blotting Analysis: A representative lot detected ionizing radiation-/IR-induced ATM Ser1981 phosphorylation in U2OS cells by Western blotting using whole cell lyates or B56γ immunoprecipitate (Shouse, G.P., et al. (2011). Oncogene. 30(35):3755-3765).
Western Blotting Analysis: A representative lot detected ionizing radiation-/IR-induced ATM Ser1981 phosphorylation in cultured primary human foreskin fibroblasts (HFFs), as well as passage-dependent basal ATM Ser1981 phosphorylation levels in HFFs (Bakkenist, C.J., et al. (2004). Cancer Res. 64(11):3748-3752).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualidade

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: 4.0 µg/mL of this antibody detected Camptothecin (Cat. No. 208925) treatment-induced increase of nuclear ATM pSer1981 foci in HeLa cells.

Descrição-alvo

350.7 kDa calculated.

forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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