MAB3408

Anti-β-Tubulin III Antibody

CHEMICON^®, mouse monoclonal, KMX-1

• Sinônimo(s): Tubulin beta-III, tubulin, beta 3, tubulin, beta 4
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• Nome do produto: Anti-Tubulin Antibody, beta, clone KMX-1, clone KMX-1, Chemicon^®, from mouse
• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: KMX-1, monoclonal
• reatividade de espécies: human, rat, mouse
• reatividade da espécie (prevista por homologia): all
• fabricante/nome comercial: Chemicon^®
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG2b
• nº de adesão NCBI: NM_006086.2
• nº de adesão UniProt: Q13509
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... TUBB3(10381)

Descrição

Descrição geral

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.

Especificidade

This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.

Imunogênio

Tubulin purified from polycephalum myxamoebae

Aplicação

Immunocytochemistry:
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.

Western blot:
0.05-0.5 μg/mL

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.

Qualidade

Routinely evaluated by Western Blot on A431 lysates.

Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.

Descrição-alvo

50 kDa

Ligação

Replaces: 04-1049

forma física

Format: Purified

Protein A purified

Purified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Armazenamento e estabilidade

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.

Nota de análise

Control
HeLa Cell lysate

A431 Cell lysate

MCF7 Cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable