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Key Documents

MAB2030

Sigma-Aldrich

Anti-Chondroitin 4 Sulfate Antibody, clone BE-123

ascites fluid, clone BE-123, Chemicon®

Sinônimo(s):

Anti-CSPG4A, Anti-HMW-MAA, Anti-MCSP, Anti-MCSPG, Anti-MEL-CSPG, Anti-MSK16, Anti-NG2

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

ascites fluid

tipo de produto de anticorpo

primary antibodies

clone

BE-123, monoclonal

reatividade de espécies

human, canine, rat, sheep, bovine, mouse

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

Isotipo

IgG1κ

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... CSPG4(1464)

Especificidade

Reacts with both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondroitin-4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans. No reaction with native proteoglycans or with proteoglycans digested with either chondroitinase AC or testicular hyaluronidase.

Imunogênio

Mouse proteoglycan.

Aplicação

Anti-Chondroitin 4 Sulfate Antibody, clone BE-123 detects level of Chondroitin 4 Sulfate & has been published & validated for use in ELISA, RIA & WB.
ELISA at 1:400-1:3,200.

RIA at 1:50-1:6,400.

Immunohistochemistry: This antibody only reacts with digested material. Fixed tissue must be digested with chondroitinase ABC (Sigma).
The Reference 23757342 should be indicated for IHC. (Reine, TM., et al., PubMed ID: 23757342)

Western Blot: 1:1000-1:4000 using ECL detection method. Chondroitinase ABC treatment of lysate sample is helpful.
In any alkali buffer, e.g. Tris or acetate (50mM or 100mM) pH 7.4-7.6. Make a stock solution by diluting one vial of Sigma Chondroitinase A, B, C, (5 units/vial) in 100 µL . For use, dilute stock 10-fold into the buffer of choice. Use at a ratio of 50-100mg of proteoglycan per 5 units of enzyme. On fixed cells, dilute in Tris acetate and on non-fixed cellsy dilute 10-25 fold in DMEM.
Poole, C.A., et al., J. Histochem. Cytochem. 1991. 39:1175-1187.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

forma física

Ascites fluid containing no preservatives.

Armazenamento e estabilidade

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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A human schwannoma cell line supports the in vitro adhesion of Plasmodium falciparum infected erythrocytes to chondroitin-4-sulfate.
K T Andrews, N K Viebig, F Wissing, N Klatt, N Oster, H Wickert, P Knolle, M Lanzer
Parasitology Research null
Cytoadherence of Plasmodium falciparum-infected erythrocytes in the human placenta.
B Maubert, N Fievet, G Tami, C Boudin, P Deloron
Parasite Immunology null
Effect of cytokine treatment on the in vitro expression of the P. falciparum adhesion receptor chondroitin-4-sulphate on the surface of human choriocarcinoma (BeWo) cells.
Yvonne Adams,Reinhard Schwartz-Albiez,James S McCarthy,Katherine T Andrews
Parasitology Research null
Efficient secretion of biologically active Chondroitinase ABC from mammalian cells in the absence of an N-terminal signal peptide.
Michael Kluppel
Molecular and Cellular Biochemistry null
Adherence of Plasmodium falciparum infected erythrocytes to CHO-745 cells and inhibition of binding by protein A in the presence of human serum.
K T Andrews, Y Adams, N K Viebig, M Lanzer, R Schwartz-Albiez
International Journal For Parasitology null

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