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Key Documents

MAB1982

Sigma-Aldrich

Anti-Integrin α6 Antibody, clone MA6

clone MA6, Chemicon®, from rat

Sinônimo(s):

CD49f

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rat

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

MA6, monoclonal

reatividade de espécies

mouse

fabricante/nome comercial

Chemicon®

técnica(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

Isotipo

IgG2κ

adequação

not suitable for Western blot

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... ITGA6(3655)

Especificidade

Reacts with the laminin receptor alpha-6beta-1 and alpha-6beta-4 complexes.

Aplicação

Immunoprecipitation, flow cytometry, immunohistochemistry in frozen tissue sections. Product does not detect alpha 6 in Western blot since the epitope most likely involves both alpha 6 and beta 1 integrin subunit. Product does not block alpha 6 integrin mediated cell adhesion to laminin. Activation of integrin signaling properties by MA6 has not been characterized. Immunoprecipitation of VLA-6 from lysate of 10E6 cell equivalent requires 2 μg of MA6. Recommended concentration for flow cytometry - 10 μg/mL, staining of frozen tissue sections - 20 μg/mL.

Optimal working dilutions must be determined by end user.

Cell lines tested by using MA6 to express (6 integrins include: mouse bone marrow-derived mast cells, melanoma cell line B16F1, squamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T lymphocytes, thymocytes and various T cell lines.
This Anti-Integrin α6 Antibody, clone MA6 is validated for use in FC, IP, IH for the detection of Integrin α6.

forma física

Format: Purified
Liquid in 0.02M Phosphate buffer, pH 7.6, with 0.25M NaCl and 0.1% sodium azide.

Armazenamento e estabilidade

Store at +2-8°C for up to 12 months.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Derivation of a new human embryonic stem cell line, Endeavour-2, and its characterization.
Kuldip S Sidhu,John P Ryan,Justin G Lees,Bernard E Tuch
In Vitro Cellular & Developmental Biology. Animal null
Lipid defect underlies selective skin barrier impairment of an epidermal-specific deletion of Gata-3.
de Guzman Strong, C; Wertz, PW; Wang, C; Yang, F; Meltzer, PS; Andl, T; Millar, SE; Ho et al.
The Journal of cell biology null
Dongren Yang et al.
The Journal of cell biology, 168(4), 655-666 (2005-02-09)
Schwann cells form basal laminae (BLs) containing laminin-2 (Ln-2; heterotrimer alpha2beta1gamma1) and Ln-8 (alpha4beta1gamma1). Loss of Ln-2 in humans and mice carrying alpha2-chain mutations prevents developing Schwann cells from fully defasciculating axons, resulting in partial amyelination. The principal pathogenic mechanism
Naomi Walsh et al.
Journal of experimental & clinical cancer research : CR, 28, 140-140 (2009-10-15)
Factors mediating the invasion of pancreatic cancer cells through the extracellular matrix (ECM) are not fully understood. In this study, sub-populations of the human pancreatic cancer cell line, MiaPaCa-2 were established which displayed differences in invasion, adhesion, anoikis, anchorage-independent growth
D M Sherry et al.
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 239(4), 278-287 (2001-07-14)
Integrin receptors mediate cell-extracellular matrix interactions and regulate many events, including cell growth, proliferation, and differentiation. Retinal integrins are incompletely understood, although these receptors are potentially important factors in normal retinal function and pathology. Immunocytochemistry was used to localize alpha

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