MAB1904

Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2

clone AL-2, Chemicon^®, from rat

• Sinônimo(s): laminin alpha1, laminin beta1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rat
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: AL-2, monoclonal
• reatividade de espécies: mouse
• fabricante/nome comercial: Chemicon^®
• técnica(s): ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NM_005559.2
• nº de adesão UniProt: P25391; P55268
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: mouse ... Lama1(16772)

Descrição

Descrição geral

Laminins are secreted glycoproteins incorporated into cell-associated extracellular matrices and an integral part of the structural scaffolding in most animal tissues. The laminin protein is made up of three polypeptide chains; α, β and γ, accounting for its flexibility in connecting various molecules. When originally characterized, laminin-1 clone AL-2 reacted with Engelbreth-Holm-Swarm (EHS) mouse laminin A & B chains on the long arm near the cross region, (Skubitz, 1987). More recently, investigations, (Schèele, 2006) have shown that the clone binds laminin β1 under reducing conditions as well as native ELISAs and that the affinity is increased by the presence of the laminin α1 chain. Thus there is significant laminin β1 reactivity with clone AL-2.

Especificidade

Other species have not been tested.

Reacts with EHS mouse laminin A & B chains on the long arm near the cross region.

Imunogênio

Epitope: Near cross region

Laminin isolated from EHS mouse sarcoma.

Aplicação

Detect Laminin-1 A&B chains using this Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2 validated for use in ELISA, IF, IH(P), IP & WB.

Immunofluorescence:
An independent laboratory (Schèele, S., 2006) has verified anti-laminin clone AL-2 to work using immunofluorescence techniques on mouse muscle tissue, including both transgenic and wild-type tissues.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Qualidade

Evaluated on a representative lot by Western blot on purified mouse laminin-1.

Descrição-alvo

~200 kDa

forma física

Format: Purified

Protein G Purified

Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.

Armazenamento e estabilidade

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable