MAB1611
Anti-Keratin Epithelial Antibody, clone AE3
clone AE3, Chemicon®, from mouse
Sinônimo(s):
Type II Cytokeratin
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified antibody
clone
AE3, monoclonal
reatividade de espécies
rat, chicken, rabbit, mouse, human, monkey, bovine
fabricante/nome comercial
Chemicon®
técnica(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotipo
IgG1
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Especificidade
Recognizes most of the basic (Type II) keratins, HMW cytokeratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. AE3 reacts with all layers of epidermis (basal layer and above). The antibody is an excellent marker for distinguishing carcinomas from non-epithelial tumours. It has been used for studying the immunolocalisation of epidermal keratins, tissue distribution of keratins, various diseases of epidermis, oral mucosa and other epithelia and other epithelia development. AE3 and AE1 (Catalog Number MAB1612) have been used extensively for distinguishing carcinoma from non-carcinoma.
Imunogênio
Human epidermal cells
Aplicação
Anti-Keratin Epithelial Antibody, clone AE3 is an antibody against Keratin Epithelial for use in WB, IH(P).
Immunohistochemistry on unfixed frozen sections or formalin-fixed paraffin-embedded tissue sections. For paraffin-embedded sections, antigen retrieval (HEIR or trypsin treatment) is strongly recommended.
Western blotting: Clone AE3 recognizes the 65 to 67, 64, 59, 58, 56 and 52 kDa human cytokeratins of the basic subfamily.
Optimal working dilutions must be determined by end user.
Western blotting: Clone AE3 recognizes the 65 to 67, 64, 59, 58, 56 and 52 kDa human cytokeratins of the basic subfamily.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytokeratins
Cytokeratins
Ligação
Replaces: 04-588
forma física
Format: Purified
Liquid in PBS. Contains 0.1% sodium azide.
Armazenamento e estabilidade
Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Nota de análise
Control
POSITIVE CONTROL: MCF-7, RT112, and A431 cell lines.
POSITIVE CONTROL: MCF-7, RT112, and A431 cell lines.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
recomendado
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Repr. 1B
Código de classe de armazenamento
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Takeuchi, K; Nagata, N; Kato, SI; Ami, Y; Suzaki, Y; Suzuki, T; Sato et al.
Journal of virology null
Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms
Spagnolo, D.V. et al.
American Journal of Clinical Pathology, 84, 697-704 (1985)
Pathogenesis of fulminant monkeypox with bacterial sepsis after experimental infection with West African monkeypox virus in a cynomolgus monkey.
Nagata, N; Saijo, M; Kataoka, M; Ami, Y; Suzaki, Y; Sato, Y; Iwata-Yoshikawa, N; Ogata et al.
International Journal of Clinical and Experimental Pathology null
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Reproduction in domestic animals = Zuchthygiene null
S R Shi et al.
The American journal of pathology, 117(1), 53-63 (1984-10-01)
Nasopharyngeal carcinoma (NPC) provides a unique opportunity to evaluate distinctive epidemiologic features and a possible etiologic relationship with Epstein-Barr virus (EBV) in human malignancy. The lack of a uniformly accepted pathologic classification for NPC has limited the application of this
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