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Merck
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Key Documents

IPFL10100

Millipore

Immobilon® -FL PVDF Membrane

10 sheets, 10 cm x 10 cm, 0.45 µm pore size, transfer membrane with low background fluorescence

Sinônimo(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

Código UNSPSC:
41105339
eCl@ss:
32031602
NACRES:
NB.22

product name

Immobilon®-FL PVDF Membrane, 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Materiais

PVDF membrane
plain filter
white filter

Nível de qualidade

Características

hydrophobic

fabricante/nome comercial

Immobilon®

técnica(s)

dot blot: suitable
western blot: suitable

filtro C × L

10 cm × 10 cm

tamanho de poro

0.45 μm pore size

capacidade

155 μg/cm2 adsorption capacity (insulin)
205 μg/cm2 adsorption capacity (BSA)
300 μg/cm2 adsorption capacity (goat IgG)

compatibilidade

for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

método de detecção

chemiluminescent
colorimetric
fluorometric

Condições de expedição

ambient

Descrição geral

The Immobilon®-FL transfer membrane is hydrophobic polyvinylidene fluoride (PVDF) microporous membrane used for the transfer of proteins from a variety of gel matrices. This transfer membrane offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. The Immobilon®-FL membrane has a nominal pore size of 0.45 μm and offers optimal blotting for proteins with molecular weights greater than 20,000 Daltons.

Aplicação

Immobilon®-FL membrane has been used in western blotting. It is also suitable for use in standard chemiluminescent or chromogenic detection.

Características e benefícios

Immobilon®-FL Membrane:
  • The first transfer membrane designed for fluorescence applications Extremely low background improves the sensitivity of all fluorescence detection protocols
  • Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
  • compatible with standard blocking agents and buffers
  • Ideal for multiplexing and chemifluorescence detections

Informações legais

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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