GF346

Transforming Growth Factor-β1 human

Animal free, >98%, recombinant, expressed in CHO cells, suitable for cell culture

• Sinônimo(s): TGFb1, TGFbI, TGFB, Transforming Growth Factor-β1
• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

Propriedades

• product name: TGF-β1,; Human Recombinant Animal Free,
• Impurezas: <0.1 μg/mg protein endotoxin (<1 EU/μg)
• Nível de qualidade: 100
• nº de adesão UniProt: P01137
• Condições de expedição: dry ice

Descrição

Descrição geral

Product Source: CHO cells

The three mammalian isoforms of TGF-β (TGF-β1, TGF-β2, TGF-β3) signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF- isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors.

Recombinant human TGF-β1 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

Aplicação

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Qualidade

Determined by TGF-β1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.

forma física

Product is presented without additives and is filtered through a 0.2 micron filter before lyophilization

Armazenamento e estabilidade

Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable