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Key Documents

AP112A

Sigma-Aldrich

Goat Anti-Human IgG Antibody, Alkaline Phosphatase conjugate

Chemicon®, from goat

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.46

fonte biológica

goat

Nível de qualidade

conjugado

alkaline phosphatase conjugate

forma do anticorpo

F(ab′)2 fragment of affinity isolated antibody

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

reatividade de espécies

human

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
western blot: suitable

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Descrição geral

Alkaline Phosphatase-conjugated Affinity Purified Goat anti-Human IgG (H+L)

Especificidade

Based on immunoelectrophoresis, the antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but the antibody may cross-react with immunoglobulins from other species.

Aplicação

Detect Human IgG using this Goat anti-Human IgG Antibody, Alkaline Phosphatase conjugate validated for use in ELISA & WB.
EIA and Western blots: 1:5,000-1:50,000.

Immunohistochemistry: 1:1,000-1:2,000.

Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Ligação

Replaces: MABN1055

forma física

Lyophilized. Buffer = 0.01 M Tirs HCl, 0.25 M NaCl, pH 8.0 with

15 mg/mL BSA and 0.05% sodium azide.

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.
Serum

Armazenamento e estabilidade

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade for better) to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

For research use only; not for use as a diagnostic.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogramas

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Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3


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Frontiers in immunology, 12, 765211-765211 (2021-12-28)
Saturation suppressor mutagenesis was used to generate thermostable mutants of the SARS-CoV-2 spike receptor-binding domain (RBD). A triple mutant with an increase in thermal melting temperature of ~7°C with respect to the wild-type B.1 RBD and was expressed in high
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Turkiye parazitolojii dergisi, 45(3), 201-206 (2021-08-05)
The follow-up of patients with cystic echinococcosis (CE) offers the opportunity of evaluating the prognosis of the infection as well as detecting relapse. This study aimed to evaluate the performance of the new multiepitope recombinant peptide (recDipol) antigen in the
Eleana Hatzidaki et al.
International journal of biochemistry and molecular biology, 11(1), 1-10 (2020-03-27)
Although monoclonal antibodies are promising, a truly fully human antibody is yet to be produced. Current human antibodies have the human sequence, but are produced in either transgenic animals or in phages. The aim of this paper was to produce

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