A3187

Anti-Human IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

• Sinônimo(s): Goat Anti-Human IgG (γ-chain specific)−AP
• Número MDL: MFCD00162443
• Código UNSPSC: 12352203
• NACRES: NA.46

Propriedades

• fonte biológica: goat
• Nível de qualidade: 200
• conjugado: alkaline phosphatase conjugate
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: secondary antibodies
• clone: polyclonal
• forma: buffered aqueous glycerol solution
• reatividade de espécies: human
• técnica(s): direct ELISA: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50; western blot: 1:30,000
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Anti-Human IgG (γ-chain specific)-Alkaline Phosphatase antibody is specific for human IgG when tested against human IgA, IgG, IgM, and Bence Jones κ, and λ myeloma proteins.

Imunogênio

Purified human IgG

Aplicação

Anti-Human IgG (γ-chain specific)-Alkaline Phosphatase antibody is suitable for use in ELISA (at 1:2500 and 1:10,000 dilutions). The antibody can also be used for western blot (1:30,000) and immunohistochemistry (at 1:50 dilutions using formalin-fixed, paraffin-embedded sections).

Proteins harvested from E. coli cultures were run on SDS-page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG (chain specific) at a concentration of 1:10000 for the secondary. IgG was detected in human serum by Elisa using alkaline phosphatase conjugated goat anti-human IgG (gamma-chain specific) diluted 1:5000 in 0.25% BSA/PBS. Assay was developed using p-nitrophenyl phosphate substrate (1mg/ml Sigma).

forma física

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable