ABE1348
Anti-Histone H2A.Z Antibody (C-term)
from rabbit, purified by affinity chromatography
Sinônimo(s):
Histone H2A.Z, H2A/z
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
purificado por
affinity chromatography
reatividade de espécies
human
técnica(s)
ChIP: suitable (ChIP-seq)
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... H2AFZ(3015)
Descrição geral
H2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
Especificidade
Recognizes histone H2A.Z
wide range expected
Imunogênio
KLH-conjugated linear peptide corresponding to region the near C-terminus of Human Histone H2A.Z.
Aplicação
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody (1μg per reaction) was used for ChIP using chromatin from HeLa and K562 cells.
ChIP-seq: A representative lot of this antibody (0.5 μg per reaction) was used to immunoprecipitate K562 chromatin for ChIP-seq analysis.
Western Blotting Analysis: A 1:2,000 dilution of a represenative lot of this antibody detected histone H2A.Z in acid extracted HeLa cell lysate.
Immunocytochemistry: A 1:500 dilution from a representative lot detected histone H2A.Z in HeLa cells.
ChIP-seq: A representative lot of this antibody (0.5 μg per reaction) was used to immunoprecipitate K562 chromatin for ChIP-seq analysis.
Western Blotting Analysis: A 1:2,000 dilution of a represenative lot of this antibody detected histone H2A.Z in acid extracted HeLa cell lysate.
Immunocytochemistry: A 1:500 dilution from a representative lot detected histone H2A.Z in HeLa cells.
This Anti-Histone H2A.Z Antibody (C-term) is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Immunocytochemistry and ELISA for the detection of Histone H2A.Z (C-term).
Qualidade
Evaluated by Western Blotting using recombinant histones
Western Blotting Analysis: A 1.5 µg/mL dilution of this antibody detected recombinant Histone H2A.Z, but not histones H1, H2A,H2A.X, H3, or H4.
Western Blotting Analysis: A 1.5 µg/mL dilution of this antibody detected recombinant Histone H2A.Z, but not histones H1, H2A,H2A.X, H3, or H4.
Descrição-alvo
~16 kDa observed
forma física
Affinity purified rabbit polyclonal in PBS containing 0.05% azide and 0.05% ProClin 300.
Outras notas
Concentration: Please refer to lot specific datasheet.
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Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Skin Sens. 1
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Gilda Stefanelli et al.
Cell reports, 36(7), 109551-109551 (2021-08-19)
Rapid removal of histone H2A.Z from neuronal chromatin is a key step in learning-induced gene expression and memory formation, but mechanisms underlying learning-induced H2A.Z removal are unclear. Anp32e was recently identified as an H2A.Z-specific histone chaperone that removes H2A.Z from
Guang Yang et al.
Nucleic acids research, 48(6), 3001-3013 (2020-01-23)
Nucleosomal histones are barriers to the DNA repair process particularly at DNA double-strand breaks (DSBs). However, the molecular mechanism by which these histone barriers are removed from the sites of DNA damage remains elusive. Here, we have generated a single
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