ABC519

Anti-FANCF Antibody

serum, from rabbit

• Sinônimo(s): Fanconi anemia group F protein, Protein FACF, FANCF
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clone: polyclonal
• application: ICC; WB
• reatividade de espécies: human
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• citations: 1

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: serum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: human
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NP_073562
• nº de adesão UniProt: Q9NPI8
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... FANCF(2188)

Descrição

Descrição geral

Fanconi anemia group F protein (FANCF), also known as Protein FACF, and encoded by the gene symbol FANCF, is a DNA repair protein that may operate in a postreplication DNA repair or may be involved in a cell cycle checkpoint function. Recent studies have implicated that FACF may play an important role in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Mutations affecting the FANCF gene have been associated with Fanconi anemia complementation group F (FANCF), a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. This disorder is also associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.

Imunogênio

GST-tagged recombinant protein corresponding to human FANCF.

Aplicação

Anti-FANCF is an antibody against FANCF for use in WB, ICC.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: A representative lot detected FANCF in 293T cells were cotransfected with HA-tagged HES1 and FA coding vectors, HeLa cell extracts treated with L685,458 , 293T cells were cotransfected with FA gene expression plasmids as indicated with either empty, or BACE1 or HES1 coding vectors, 293T cells were cotransfected with FANCF expression plasmids along with increasing amounts of HES1 coding vector (Tremblay, C.S., et al. (2008). Blood Journal. 112:P2062-2070).
Immunocytochemistry Analysis: A representative lot detected FANCF in HeLa cells treated with the gamma-secretase inhibitor L685,458 (Tremblay, C.S., et al. (2008). Blood Journal. 112:P2062-2070).

Qualidade

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected FANCF in 10 µg of MCF-7 cell lysate.

Descrição-alvo

~45 kDa observed

forma física

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1