ABC137

Anti-ATPIF1 Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): ATPase inhibitor, mitochondrial, Inhibitor of F(1)F(o)-ATPase, IF(1), IF1, ATPIF1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human, mouse
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NP_057395
• nº de adesão UniProt: Q9UII2
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... ATPIF1(93974)

Descrição

Descrição geral

ATPIF1 is an interesting protein that acts on ATP synthase and prevents it from catabolizing ATP when the mitochondrial membrane potential falls below a threshold, which ATP synthase starts to do by hydrolyzing ATP to pump protons out of the mitochondrial matrix to restore ion balance. ATPIF1 also indirectly regulates heme synthesis by modulating the mitochondrial pH and redox potential. ATPIF1 is expressed in the mitochondria and its active is determined by conformation. Homodimers are active, homotetramers are inactive and their formation is governed by pH. ATPIF1, because of its effects on ATP and heme, is important in cellular development, heme biosynthesis, energy metabolism and angiogenesis and erythrocytes differentiation.

Imunogênio

Epitope: N-terminal inhibitory region & Antiparallel alpha-helical coiled region

GST-tagged recombinant protein corresponding to the N-terminal inhibitory region & Antiparallel alpha-helical coiled eegion of human ATPIF1.

Aplicação

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-ATPIF1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of ATPIF1.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected ATPIF1 in 10 µg of HEK293 cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected ATPIF1 in HeLa, A431, and NIH/3T3 cells.

Qualidade

Evaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected ATPIF1 in 10 µg of T47D cell lysate.

Descrição-alvo

~12 kDa observed. Uncharacterized band(s) may appear in some lysates.

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable