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Key Documents

AB3468

Sigma-Aldrich

Anti-Heat Shock Protein 90β Antibody

Chemicon®, from rabbit

Sinônimo(s):

HSP84

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

reatividade de espécies

human, rat, mouse

fabricante/nome comercial

Chemicon®

técnica(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

Especificidade

Reacts with a protein of 84 kD identified as heat shock protein 90beta (HSP90beta). No reactivity with HSP90alpha/HSP86.

CELLULAR LOCALIZATION: Nuclear and Cytoplasmic.

Imunogênio

Synthetic peptide corresponding to amino acids 2-13 from the N-terminus of mouse heat shock protein 90b.

Aplicação

Immunoblotting: 5 μg/mL

Immunohistochemistry (frozen and formalin/paraffin): 5-10 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Immunocytochemistry

Immunoprecpitation: 10 μg/mg of protein lysate.

Optimal working dilutions must be determined by end user.
Research Category
Protein Trafficking
Research Sub Category
Chaperones
This Anti-Heat Shock Protein 90β Antibody is validated for use in IP, WB, IC, IH(P) for the detection of Heat Shock Protein 90β.

forma física

Format: Purified
Purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.

Armazenamento e estabilidade

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Nota de análise

Control
POSITIVE CONTROL: MAD109 cells or breast carcinoma.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Malignant transformation in a defined genetic background: proteome changes displayed by 2D-PAGE.
Putz, SM; Vogiatzi, F; Stiewe, T; Sickmann, A
Molecular Cancer null
Detection of anti-heat shock protein 90 beta (Hsp90beta) antibodies in cerebrospinal fluid.
C Cid, M Garcia-Villanueva, M Salinas, A Alcazar
Journal of Immunological Methods null
Signalling profile and antitumour activity of the novel Hsp90 inhibitor NVP-AUY922 in multiple myeloma.
Stuhmer, T; Zollinger, A; Siegmund, D; Chatterjee, M; Grella, E; Knop, S; Kortum et al.
Leukemia null
Ying-Li Wu et al.
Journal of cell science, 119(Pt 13), 2797-2806 (2006-06-15)
The involvement of telomerase in cellular immortalization and senescence has often been assessed by means of telomerase expression at the RNA level and quantification of telomerase activity by the telomeric repeat amplification protocol assay. However, these methods either neglected the
Heat shock protein 90 overexpression independently predicts inferior disease-free survival with differential expression of the alpha and beta isoforms in gastrointestinal stromal tumors.
Li, CF; Huang, WW; Wu, JM; Yu, SC; Hu, TH; Uen, YH; Tian, YF; Lin, CN; Lu, D; Fang, FM; Huang, HY
Clinical cancer research : an official journal of the American Association for Cancer Research null

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