70796-M

His•Tag^® Monoclonal Antibody

Novagen^®

• Sinônimo(s): Anti-His antibody
• Código UNSPSC: 12352203
• NACRES: NA.43

Propriedades

• forma do anticorpo: purified antibody
• Nível de qualidade: 100
• fabricante/nome comercial: Novagen^®
• condição de armazenamento: OK to freeze
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

Sensitive, specific detection of His•Tag fusion proteins

The HIS TAG^® Monoclonal Antibody is a mouse monoclonal antibody (IgG₁) directed against the HIS TAG^® sequence encoded by many of the NOVAGEN^® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (K_d = 5 × 10^-8 to 1 × 10^-9 M) enables sensitive, specific detection of HIS TAG^® fusion proteins at antibody concentrations of 0.1-0.2 g/ml. The HIS TAG^® Monoclonal Antibody binds to N-terminal, C-terminal, and internal HIS TAG^® sequences. This antibody also detects the recombinant marker bands in the Perfect Protein<TMSYMBOL></TMSYMBOL> and Trail Mix<TMSYMBOL></TMSYMBOL> Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-g package size provides enough purified antibody for 1000 mL of working solution.

* Not recommended for HRP-based detection of Trail Mix Western Markers.

Advertência

Toxicity: Standard Handling (A)

Informações legais

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable