11965085001
Roche
Anti-His6-Peroxidase
from mouse IgG1
Sinônimo(s):
antibody
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About This Item
Código UNSPSC:
12352203
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
peroxidase conjugate
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
BMG-his-1, monoclonal
forma
lyophilized
embalagem
pkg of 50 U
fabricante/nome comercial
Roche
Isotipo
IgG1
temperatura de armazenamento
2-8°C
Descrição geral
Anti-His6-Peroxidase is a monoclonal antibody to His6-tagged proteins, conjugated to horseradish peroxidase. Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the epitope-sequence location; however, it preferentially recognizes the C-terminal His6 epitope with high sensitivity.
Especificidade
Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the epitopesequence. However, Anti-His6-Peroxidase preferentially recognizes the C-terminal His6 epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used.
The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.
Aplicação
Anti-His6-Peroxidase has been used in in vitro serum stability of fusion toxins, pull-down assay and western blotting.
Anti-His6-Peroxidase is used for the detection of His6-tagged proteins in:
- ELISA (enzyme-linked immunosorbent assay)
- Western blot
Qualidade
Function test: Western blot using extracts from cell line expressing a recombinant His6-tagged protein.
Nota de preparo
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- ELISA: 100 mU/ml
- Western blot: 100mU/ml
Reconstituição
Add 1 ml double-distilled water to a final concentration of 50 U/ml.
Reconstitution should be performed for at least 10 minutes.
Reconstitution should be performed for at least 10 minutes.
Outras notas
For life science research only. Not for use in diagnostic procedures.
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Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Skin Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
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