70181-M

NovaBlue Singles Competent Cells - Novagen

Escherichia coli, rod shaped

• Sinônimo(s): Competent E. coli Cells
• Código UNSPSC: 41106202

Propriedades

• product name: NovaBlue Singles Competent Cells - Novagen, NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.
• fonte biológica: Escherichia coli
• Nível de qualidade: 100
• fabricante/nome comercial: Novagen^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• modo de crescimento: adherent or suspension
• morfologia: rod shaped
• técnica(s): microbiological culture: suitable
• transformação celular: transformation efficiency: >1.5×10⁸ cfu/μg
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Genotype:

endA1 hsdR17 (r_K12^– m_K12⁺) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA⁺B⁺ lacI^qZΔM15::Tn10] (Tet^R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.

NovaBlue competent cells are for routine molecular cloning applications. NovaBlue is a K-12 strain derivative that offers high transformation efficiency,facilitates plasmid stability, and allows blue/whitescreening with appropriate plasmids.

NovaBlue Singles Competent Cells, like all Novagen Singles Competent Cells, are designed for ultimate convenience and reliability in plasmid transformation. The cells are grown and made chemically competent by an optimized procedure. Cells are provided in 50-l volumes that eliminate the need to aliquot, freeze/thaw, or waste partially used vials, thus saving time and increasing performance. For use, simply thaw, add DNA, incubate five minutes on ice, heat shock for 30 seconds, place on ice for two minutes, add SOC Medium, and plate directly (when selecting for ampicillin resistance) or after incubation at 37°C for 30 minutes (when selecting for kanamycin resistance).

Componentes

•11 or 22Singles Competent Cells•2 or 4 × 2 mlSOC Medium•10 µlTest Plasmid

Advertência

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Perfil do DNA

Genotype: endA1 hsdR17 (r_K12^– m_K12⁺) supE44 thi-1 recA1 gyrA96 relA1 lac Fʹ[proA⁺B⁺ lacI^qZΔM15::Tn10] (Tet^R)

Informações legais

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable