71059-M

RosettaBlue(DE3) Competent Cells - Novagen

Escherichia coli, rod shaped

• Código UNSPSC: 41106202
• NACRES: NA.81

Propriedades

• product name: RosettaBlue(DE3) Competent Cells - Novagen, RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
• fonte biológica: Escherichia coli
• Nível de qualidade: 100
• fabricante/nome comercial: Novagen^®
• condição de armazenamento: OK to freeze
• modo de crescimento: adherent or suspension
• morfologia: rod shaped
• técnica(s): microbiological culture: suitable
• transformação celular: transformation efficiency: >1×10⁸ cfu/μg
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Genotype: endA1 hsdR17 (r_K12^– m_K12⁺) supE44 thi-1 recA1 gyrA96 relA1 lac (DE3) F′[proA⁺B⁺ lacI^qZΔM15::Tn10] pRARE (Cam^R, Tet^R)





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RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and recA endAlacI^q mutations with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, and GGA on a chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. Blue/white screening is not possible with RosettaBlue(DE3) strains due to the presence of the lacZ α-peptide coding sequence in the λDE3 prophage.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.

Componentes

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosettaBlue(DE3) Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Advertência

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Informações legais

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany