616376

TRAIL, Human, Recombinant, NSO cells

• Sinônimo(s): Apo2L; TNF-Related Apoptosis-Inducing Ligand
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• recombinante: expressed in NSO cells
• Nível de qualidade: 100
• Ensaio: ≥97% (SDS-PAGE)
• forma: lyophilized
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• Impurezas: ≤1 EU/μg Endotoxin (EU/μg TRAIL)
• Condições de expedição: ambient
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Recombinant, human TRAIL (amino acids 95-281) with an amino-terminal 6X histidine tag and expressed in NSO cells. A type II transmembrane protein with a C-terminal extracellular domain that exhibits homology to other members of this tumor necrosis factor (TNF) family. Displays potent anti-tumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant bioactive TRAIL is a non-disulfide-linked homotrimer but may contain a small amount (up to 10%) of disulfide-linked homodimers.

Recombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues.

Ações bioquímicas/fisiológicas

ED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D

Advertência

Toxicity: Standard Handling (A)

forma física

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.

Reconstituição

Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to ≥20 µg/ml in sterile PBS, 0.1% BSA.

Outras notas

Griffith, T.S., et al. 1999. J. Exp. Med.189, 1343.
Mariani, S.M., et al. 1997. J. Cell Biol.137, 221.
Pan, G., et al. 1997. Science277, 815.
Pan, G., et al. 1997. Science276, 111.
Sheridan, J.P., et al. 1997. Science277, 818.
Pitti, R.M., et al. 1996. J. Biol. Chem.271, 12687.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable