SRP8024

TRAIL, Soluble human

recombinant, expressed in E. coli, His tagged, >98% (SDS-PAGE)

• Sinônimo(s): Apo-2L, CD253, CD339, TRAIL
• Código UNSPSC: 12352200
• NACRES: NA.77

Propriedades

• fonte biológica: human
• recombinante: expressed in E. coli
• etiqueta: His tagged
• Ensaio: >98% (SDS-PAGE)
• Formulário: liquid
• peso molecular: ~26 kDa
• embalagem: pkg of 20 μg
• concentração: ≥0.2 mg/mL
• técnica(s): activity assay: suitable; cell culture | mammalian: suitable; transfection: suitable
• Impurezas: <0.01 EU/μg endotoxin, tested
• cor: clear
• nº de adesão UniProt: P50591
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... TNFSF10(8743)

Descrição

Descrição geral

TNF-related apoptosis-inducing ligand (TRAIL; Apo2L; CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines, TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. The gene encoding TRAIL is localized on human chromosome 3q26.31.

Aplicação

TRAIL, soluble human has been used in cell culture, chemical transfection and in caspase-3/7 activity assay.

Ações bioquímicas/fisiológicas

Trimerized TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis upon ligation of cell surface TRAIL-receptor 1 (R1) and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-receptors 1,-2,-3,-4 {TRAIL-R1 (DR4; Apo2; CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A; TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1; LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D)} and osteoprotegerin (OPG; OCIF; TNFRSF11B).

forma física

Solution in 20 mM HEPES, pH 7.9, 120 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT.

Outras notas

The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable