407294

αA-Interferon, Human, Recombinant, E. coli

• Sinônimo(s): IFNA2A, IFNA2B, IFNA2C, rhIFN-αA
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• Ensaio: ≥95% (SDS-PAGE)
• Nível de qualidade: 100
• forma: liquid
• atividade específica: ≥2.8 x 10⁸ units/mg protein; ≥5 x 10⁶ units/mL
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• técnica(s): cell based assay: suitable
• nº de adesão NCBI: NM_000605.4
• nº de adesão UniProt: P01563
• Condições de expedição: wet ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... IFNA1(3340)

Descrição

Descrição geral

Research area: Immunoand cks

Interferons are cytokines secreted by mammalian cells. They are broadly divided into type I– interferon alpha (IFN-α) and interferon beta (IFN-β), type II – interferon-gamma (IFN-γ), and type III – interferon lambda (IFN-λ) based on their structure, function, and receptor specificity. Recombinant αA interferon is a hydrophobic protein belonging to the IFN-α family of proteins.

Aplicação

Interferon alpha has been used to evaluate its effect on VSV (vesicular stomatitis virus) infection in pancreatic ductal adenocarcinoma (PDA) cell lines and study the relationship between type I IFNsignaling, resistance to VSV infection, and the JAK/STAT (Janus kinase/signal transducers and activators of transcription) signaling pathways in PDA cell lines. It has also been used to study the inhibitory effect of Morbillivirus V Proteins on Interferon Signaling Pathways.

Ações bioquímicas/fisiológicas

Recombinant, human αA-interferon expressed in E. coli have antiviral, antiproliferative, antineoplastic, and immunomodulating properties. Recombinant interferon αA (IFN-αA) is used as the first line of treatment for hepatitis C. It also displays a great therapeutic potential with chronic viral hepatitis, polycythemia, leukemia, and various other malignancies. It enhances natural killer cell activity, suppresses bone marrow function, and induces apoptotic cell death in non-melanoma skin cancer.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit of interferon per ml of medium will cause a 50% inhibition of VSV-induced cytopathic effect on bovine MDBK cells. Units are determined with respect to the NIH international reference standard for human αA-IFN. Dilute in aqueous buffer containing at least 0.1% BSA or HSA.

forma física

In PBS containing 0.1% BSA.

Nota de preparo

Dilute only in buffers containing protein such as 0.1% BSA.

Reconstituição

Following initial thaw, aliquot and freeze (-70°C).

Outras notas

Fujishima, H., et al. 1998. Int. J. Cancer 76, 423.
Haria, M., and Benfield, P. 1995. Drugs 50, 873.
Familletti, P.C., et al. 1981. Methods Enzymol. 78, 387.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable