371955-M

H-7, Dihydrochloride - CAS 108930-17-2 - Calbiochem

A broad-based, cell-permeable, reversible, ATP-competitive serine/threonine kinase inhibitor.

• Sinônimo(s): H-7, Dihydrochloride - CAS 108930-17-2 - Calbiochem, 1-(5-Isoquinolinesulfonyl)-2-methylpiperazine, 2HCl
• Fórmula empírica (Notação de Hill): C₁₄H₁₇N₃O₂S · 2HCl
• Número CAS: 108930-17-2
• Peso molecular: 364.29
• Número MDL: MFCD00036961
• Código UNSPSC: 12352200

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥98% (TLC)
• forma: lyophilized solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; protect from light
• cor: light yellow
• solubilidade: water: 20 mg/mL
• temperatura de armazenamento: 2-8°C
• InChI: 1S/C14H17N3O2S.2ClH/c1-11-9-16-7-8-17(11)20(18,19)14-4-2-3-12-10-15-6-5-13(12)14;;/h2-6,10-11,16H,7-9H2,1H3;2*1H
• chave InChI: OARGPFMFRLLKPF-UHFFFAOYSA-N

Descrição

Descrição geral

A broad-based, cell-permeable, reversible, ATP-competitive serine-threonine kinase inhibitor. Potent inhibitor of myosin light chain kinase (K_i = 97 µM), protein kinase A (K_i = 3.0 µM), protein kinase C (K_i = 6 µM), and protein kinase G (K_i = 5.8 µM). Induces apoptotic DNA fragmentation and cell death in HL-60 human promyelocytic leukemia cells. Also inhibits telomerase activity in quercetin-, H-89-, or herbimycin A-treated NPC-076 cells.

A broad-based, cell-permeable, reversible, ATP-competitive serine/threonine kinase inhibitor. Inhibits protein kinase A (K_i = 3.0 µM), myosin light chain kinase (K_i = 97 µM), protein kinase C (K_i = 6.0 µM), and protein kinase G (K_i = 5.8 µM). Induces apoptotic DNA fragmentation and cell death in HL-60 human promyelocytic leukemia cells. Also inhibits telomerase activity in quercetin-, H-89-, or herbimycin A-treated NPC-076 cells.

Ações bioquímicas/fisiológicas

Primary Target
MLCK

Target K_i: 3.0 µM, 97 µM, 6.0 µM, 5.8 µM against protein kinase A , myosin light chain kinase, protein kinase C , and protein kinase G, respectively

Advertência

Toxicity: Standard Handling (A)

Nota de preparo

Further dilute with aqueous buffers just prior to use.

Reconstituição

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C.

Outras notas

Ku, W.-C., et al. 1997. Biochem. Biophys. Res. Commun. 241, 730.
Jarvis, W.D., et al. 1994. Cancer Res.54, 1707.
Bergstrand, H., et al. 1992. J. Pharmacol. Exp. Therap.263, 1334.
Gimond, C., et al. 1992. Exp. Cell Res.203, 365.
Quick, J., et al. 1992. Biochem. Biophys. Res. Commun.187, 657.
Bouli, N.M., and Davis, M. 1990. Brain Res.525, 198.
Takahashi, I., et al. 1990. J. Pharmacol. Exp. Ther.255, 1218.
Schachtele, C., et al. 1988. Biochem. Biophys.Res. Commun.151, 542.
Kawamoto, S., and Hidaka, H. 1984. Biochem. Biophys. Res. Commun.125, 258.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable