362185

N-Glycosidase F, Elizabethkingia meningosepticum

• Sinônimo(s): N-Glycosidase F, Elizabethkingia meningosepticum, Glycopeptidase F, PNGase F
• Número CAS: 83534-39-8
• Número da licença da enzima: 3.5.1.52 (BRENDA, IUBMB)
• Número MDL: MFCD00131222
• Código UNSPSC: 12352202
• NACRES: NA.54

Propriedades

• fonte biológica: bacterial (Elizabethkingia meningosepticum)
• Nível de qualidade: 100
• conjugado: (N-linked)
• Formulário: liquid
• atividade específica: ≥20,000 units/mg protein; ≥4500 units/mL
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: do not freeze
• atividade externa: Proteases, none detected
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1-3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.

forma física

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Outras notas

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable