G5166
PNGase F from Elizabethkingia miricola
buffered aqueous solution
Sinônimo(s):
N-Glycosidase F, Peptide N-glycosidase
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.32
Produtos recomendados
fonte biológica
bacterial (Elizabethkingia miricola)
Nível de qualidade
conjugado
(N-linked)
Formulário
buffered aqueous solution
atividade específica
≥20000 units/mg protein
peso molecular
36 kDa
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
Descrição geral
PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.
Aplicação
PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.
Used to deglycosylate protein.
Ações bioquímicas/fisiológicas
Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
Definição da unidade
One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.
forma física
Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Escolha uma das versões mais recentes:
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
A novel humanized GLP-1 receptor model enables both affinity purification and Cre-LoxP deletion of the receptor
Jun LS, et al.
PLoS ONE, 9(4), e93746-e93746 (2014)
Ying-Nai Wang et al.
STAR protocols, 3(1), 101198-101198 (2022-03-05)
Immunotherapy via PD-1/PD-L1 blockade is a promising strategy to eradicate cancer cells. However, the PD-L1 pathological level is inconsistent with the therapeutic response and is not a reliable biomarker to stratify patients for anti-PD-1/PD-L1 therapy. Here, we describe patient sample deglycosylation
Identification and Characterization of a Novel Prokaryotic Peptide N-GLYCOSIDASE FROM ELIZABETHKINGIA MENINGOSEPTICA
Sun G, et al.
The Journal of Biological Chemistry, 290(12), 7452-7462 (2015)
Lucy S Jun et al.
PloS one, 9(4), e93746-e93746 (2014-04-04)
Class B G protein-coupled receptors (GPCRs) are important regulators of endocrine physiology, and peptide-based therapeutics targeting some of these receptors have proven effective at treating disorders such as hypercalcemia, osteoporosis, and type 2 diabetes mellitus (T2DM). As next generation efforts
Gyoung Nyoun Kim et al.
PLoS pathogens, 17(12), e1010092-e1010092 (2021-12-17)
The development of safe and effective vaccines to prevent SARS-CoV-2 infections remains an urgent priority worldwide. We have used a recombinant vesicular stomatitis virus (rVSV)-based prime-boost immunization strategy to develop an effective COVID-19 vaccine candidate. We have constructed VSV genomes
Artigos
N-Linked Glycan Strategies; Sigma-Aldrich.com
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica