362300

N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli

• Sinônimo(s): N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli, Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine Amidase, PNGase F, Glycopeptidase F
• Número CAS: 83534-39-8
• Número da licença da enzima: 3.5.1.52 (BRENDA, IUBMB)
• Número MDL: MFCD00131222
• Código UNSPSC: 12352202
• NACRES: NA.54

Propriedades

• recombinante: expressed in E. coli
• Nível de qualidade: 100
• forma: liquid
• atividade específica: ≥10 units/mg protein
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• atividade externa: β-N-acetylhexosaminidase, α-fucosidase, β-galactosidase, α- and β-mannosidase, proteases, none detected
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Note: 1 mU = 1 milliunit.

Recombinant, Chryseobacterium meningosepticum N-Glycosidase F expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of N-glycans from high mannose, hybrid and complex glycopeptides, and from glycoproteins generating asparagine-linked oligosaccharides.

Recombinant, Chryseobacterium meningosepticum N-Glycosidase F expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of N-glycans from high mannose, hybrid and complex glycopeptides, and from glycoproteins generating asparagine-linked oligosaccharides. Supplied with a series of buffers.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit is defined as the amount of enzyme that will completely catalyze the release of N-linked oligosaccharides from 1 µmol denatured ribonuclease in 1 min at 37°C, pH 7.5.

forma física

Enzyme supplied in 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Reconstituição

Following initial thaw, aliquot and freeze (-20°C).

Outras notas

Fan, J.-Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Altmann, F., et al. 1995. Glycoconj. J. 12, 84.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable