345749

GDF-8 Propeptide

≥90% (SDS-PAGE), lyophilized powder, mouse

• Código UNSPSC: 12352203
• NACRES: NA.26

Propriedades

• product name: GDF-8 Propeptide, His•Tag^®, Mouse, Recombinant, GDF-8 Propeptide, His•Tag, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.
• recombinante: expressed in mouse NSO cells (myeloma cells)
• Nível de qualidade: 100
• Ensaio: ≥90% (SDS-PAGE)
• forma: lyophilized
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• Impurezas: ≤1.0 EU/μg protein Endotoxin
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Recombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.

Aplicação

GDF-8 Propeptide, His•Tag, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.

Ações bioquímicas/fisiológicas

ED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.

Advertência

Toxicity: Standard Handling (A)

forma física

Lyophilized from 0.2 µm filtered PBS, BSA.

Reconstituição

Following reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 50 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Outras notas

Hill, J.J. et al. 2003. Molecular Endocrinology17, 1144.
Thies, R.S. et al. 2001. Growth Factors18, 251.
Chang, H. et al. 2002. Endocrine Reviews23, 787.
McPherron, A.C. et al. 1997. Nature387, 83.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2