SRP4623P

Myostatin Proform human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Sinônimo(s): GDF-8, Growth Differentiation Factor 8, MSTN, MSTN Muscle Hypertrophy
• Número MDL: MFCD03457656
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in E. coli
• Ensaio: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Formulário: lyophilized
• peso molecular: ~27.8 kDa
• embalagem: pkg of 10 μg
• Impurezas: endotoxin, tested
• nº de adesão NCBI: O14793
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... MSTN(2660)

Descrição

Descrição geral

MSTN (myostatin) belongs to the transforming growth factor-β (TGF β) superfamily containing numerable homologous cytokine proteins. This gene is expressed in skeletal muscle, and codes for a precursor protein composed of an N-terminal leader peptide, a pro-peptide domain, and a C-terminal domain. This precursor protein is proteolytically cleaved by bone morphogenetic protein 1 (BMP-1) at a conserved four amino acid RSRR site to produce the 13kDa mature protein.

Ações bioquímicas/fisiológicas

MSTN (myostatin) functions as a negative modulator of muscle cell development. It functions through forming heteromeric receptor complexes such as with activin type II receptor B (ActRIIB), and with lesser affinity with ActIIA. This interaction results in the recruitment of type I receptors like activin receptor-like kinase 4 (ALK4) or ALK5, leading to the phosphorylation of Smad2 (small mothers against decapentaplegic) and Smad3, which move to the nucleus and suppress myogenesis. In MSTN-null mice, there is delayed skin wound healing due to the increased expression of decorin, an endogenous transforming growth factor-β (TGF β) suppressor. Topical treatment with TGF-β1 restored skin wound healing, suggesting that MSTN agonists might have therapeutic beneficial in skin wound repair.

forma física

Lyophilized without any additives.

Reconstituição

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1 to 1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable