234567M

Concanavalin A, Canavalia ensiformis

Concanavalin A, Canavalia ensiformis, CAS 11028-71-0, is a carbohydrate-binding protein that agglutinates various animal cells. Restores the growth pattern of tumor cells to that of normal cells.

• Sinônimo(s): Concanavalin A, Canavalia ensiformis, Con A, Jack Bean Hemagglutinin
• Número CAS: 11028-71-0
• Número MDL: MFCD00071069
• Código UNSPSC: 12352202
• NACRES: NA.42

Propriedades

• Nível de qualidade: 100
• descrição: Merck USA index - 14, 2495
• Formulário: lyophilized
• potência: ≤20 μg/mL lectin agglutination activity (as determined from serial dilutions of a 1 mg/mL solution)
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• pI: 4.5 - 5.5
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Carbohydrate-binding protein that agglutinates various animal cells. Also known to restore the growth pattern of tumor cells to that of normal cells. T lymphocyte activator. Has binding sites for metal ions, α-D-mannose, and α-D-glucose. Has a pI of 4.5-5.5.

Advertência

Toxicity: Harmful (C)

Nota de preparo

To dissolve, add Con A slowly to a solution of 10 mM HEPES buffered saline, 100 µM CaCl₂, pH 8.5. Allow the lectin to dissolve without agitation.

Reconstituição

Following reconstitution, aliquot and freeze (-20°C) or add 0.08% NaN₃ and refrigerate (4°C). Stock solutions are stable for up to 6 months at -20°C or for up to 3 months at 4°C.

Nota de análise

The agglutination activity is the lowest conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25°C; requires type A human blood.

Outras notas

Gantner, F., et al. 1995. Hepatology 21, 190.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317,H334,H361
• Declarações de precaução: P201 - P202 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313
• Classificações de perigo: Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable