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1.50247

Supelco

Purospher® STAR NH2 (5 µm)

L × I.D. 150 mm x 4.6 mm LiChroCART® HPLC cartridge, manuCART holder required for use

Sinônimo(s):

Purospher® STAR NH2 (5 µm) LiChroCART® 150-4.6

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About This Item

Código UNSPSC:
41115709

Materiais

stainless steel column

Nível de qualidade

Agency

suitable for USP L8

linha de produto

Purospher®

Características

endcapped: no

embalagem

1 ea of

Extensão da rotulagem

3.5% Carbon loading

Parâmetros

250 bar pressure
60 °C max. temp.

técnica(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)

coluna C × D.I.

15 cm × 4.6 mm

área da superfície

330 m2/g

matriz

fully porous particle

Grupo ativo da matriz

NH2 bonding phase

tamanho de partícula

5 μm

tamanho de poro

120 Å pore size

operating pH range

2-7.5

técnica de separação

hydrophilic interaction (HILIC)
normal phase
reversed phase

temperatura de armazenamento

no temp limit

Descrição geral

Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phaseconsisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm LiChroCART® 150-4.6 is a cartridge column which requires a manuCART cartridge column holder part number 1.51486.0001.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART® 4-4 part number 1.50267.0001. LiChroCART® cartridge columns mounted with a manuCART cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar.The column dimension of 150 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Aplicação

Intended to be used in the separation of alcohols,carboxylic acids, fat soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water soluble vitamins.

Características e benefícios

  • Ultra-pure silica (99.999%)
  • Low silanol activity
  • Reproducible results from run-to-run and batch-to-batch

Nota de análise

Pressure: ≤ 45 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): ≤ 3.0

Informações legais

LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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