14-452-M

PDK1 Protein, active, 10 µg

Active, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.

• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.26

Propriedades

• fonte biológica: human
• Nível de qualidade: 100
• recombinante: expressed in baculovirus infected Sf21 cells
• linha de produto: Upstate^®
• atividade específica: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• peso molecular: M_w 59 kDa
• purificado por: (Ni²⁺/NTA-agarose)
• reatividade de espécies: human
• fabricante/nome comercial: Upstate^®
• técnica(s): activity assay: suitable (kinase)
• nº de adesão: (FUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. ); NM_002613.3
• nº de adesão NCBI: NM_002613.3; NM_031268.4
• nº de adesão UniProt: O15530
• Informações sobre genes: human ... PDPK1(5170)

Descrição

Descrição geral

N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end.

Ações bioquímicas/fisiológicas

Protein Target: PDK1

Target Sub-Family: AGC

Embalagem

Also available in 10 μg size and in bulk

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-452M when ordering the 250 µg size.

Qualidade

Routinely evaluated by phosphorylation of PDKtide

Descrição-alvo

PDK1

forma física

50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 0.1% 2-mercaptoethanol, 1mM benzamidine, 200μM PMSF and 5% glycerol

Ni²⁺/NTA-agarose

Armazenamento e estabilidade

6 months at -70°C

Outras notas

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317
• Declarações de precaução: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classificações de perigo: Skin Sens. 1
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°C): Not applicable