126575
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free
Sinônimo(s):
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free
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About This Item
Produtos recomendados
descrição
Merck USA index - 14, 8468
Ensaio
≥95% (cellulose acetate electrophoresis)
Formulário
powder
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
Impurezas
≤0.02% fatty acids
≤3% ash
≤5% moisture
cor
white to light yellow
light yellow to light brown
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
Categorias relacionadas
Descrição geral
Albumin, Bovine Serum, (BSA) Fraction V, Fatty Acid-Free is useful for immunodiagnostic assays. It is designed for use in serological testing, radioimmunoassay, and hormone response studies. It is also applicable in binding and transport studies. BSA is prepared by cold ethanol extraction (Cohn method) with a pH: 6.8-7.4.
Bovine serum albumin (BSA) is a non-glycosylated globular, α-helical protein belonging to the serum albumin family. It consists of three domains with two sub-domains each and has 17-disulfide bonds.
Aplicação
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free has been used:
- as a component of: blocking buffer immunoelectron microscopy (IEM) for various cells
- mitochondrial isolation buffer (MIB) and mitochondria assay solution (MAS) to trap free fatty acids released during adipose tissue homogenization
- blocking buffer for wholemount staining of the esophageal epithelium (EE) in immunofluorescence
Ações bioquímicas/fisiológicas
Bovine Serum Albumin (BSA), the most vital component of cell culture media, aids in embryonic stem cells (hESC) differentiation. It aids the transportation of drugs, hormones, and fatty acids. BSA is also used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
Advertência
Toxicity: Standard Handling (A)
Nota de preparo
May take up to 24 h to fully dissolve.
Reconstituição
Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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