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Merck
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Documentos Principais

07-595

Sigma-Aldrich

Anti-acetyl-Histone H4 (Lys12) Antibody

serum, Upstate®

Sinônimo(s):

H4K12Ac, Histone H4 (acetyl K12)

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

serum

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

reatividade de espécies

Saccharomyces cerevisiae, human

fabricante/nome comercial

Upstate®

técnica(s)

ChIP: suitable (ChIP-seq)
dot blot: suitable
western blot: suitable

Isotipo

IgG

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

acetylation (Lys12)

Informações sobre genes

Descrição geral

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Especificidade

Broad species cross-reactivity expected
Recognizes Histone H4 acetylated on lysine 12.

Imunogênio

peptide containing the sequence (LGAcKGG) corresponding to lysine 12 acetylation of yeast Histone H4.

Aplicação

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-acetyl-Histone H4 (Lys12) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, DB, WB, ChIP-seq to detect acetyl-Histone H4 (Lys12) also known as H4K12Ac, Histone H4 (acetyl K12).

Qualidade

routinely evaluated by immunoblot on acid extracted proteins from HeLa cells

Descrição-alvo

10 kDa

forma física

Depleted rabbit antiserum in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Immunodepleted Serum

Armazenamento e estabilidade

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análise

Control
Acid extracted proteins from HeLa cells treated with sodium butyrate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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The molecular topography of silenced chromatin in Saccharomyces cerevisiae.
Thurtle, DM; Rine, J
Genes & Development null
Histone deacetylase inhibitors modify pancreatic cell fate determination and amplify endocrine progenitors.
Haumaitre, Cecile, et al.
Molecular and cellular biology, 28, 6373-6383 (2008)
The complex pattern of epigenomic variation between natural yeast strains at single-nucleosome resolution.
Filleton, F; Chuffart, F; Nagarajan, M; Bottin-Duplus, H; Yvert, G
Epigenetics & Chromatin null
Yohan Suryo Rahmanto et al.
The Journal of biological chemistry, 291(18), 9690-9699 (2016-03-10)
ARID1A is a tumor suppressor gene that belongs to the switch/sucrose non-fermentable chromatin remodeling gene family. It is mutated in many types of human cancer with the highest frequency in endometrium-related ovarian and uterine neoplasms including ovarian clear cell, ovarian
Schizosaccharomyces pombe Hat1 (Kat1) is associated with Mis16 and is required for telomeric silencing.
Tong, K; Keller, T; Hoffman, CS; Annunziato, AT
Eukaryotic Cell null

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