760749

Dibenzocyclooctyne-PEG4-biotin conjugate

for Copper-free Click Chemistry

• Sinônimo(s): Polyethylene glycol, DBCO-PEG4-biotin
• Fórmula empírica (Notação de Hill): C₃₉H₅₁N₅O₈S
• Número CAS: 1255942-07-4
• Peso molecular: 749.92
• Número MDL: MFCD22572661
• Código UNSPSC: 12352003
• ID de substância PubChem: 329766893
• NACRES: NA.22

Propriedades

• forma: solid
• adequação da reação: reaction type: click chemistry
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)NCCOCCOCCOCCOCCC(=O)NCCC(=O)N3Cc4ccccc4C#Cc5ccccc35
• InChI: 1S/C39H51N5O8S/c45-35(12-6-5-11-34-38-32(28-53-34)42-39(48)43-38)41-18-20-50-22-24-52-26-25-51-23-21-49-19-16-36(46)40-17-15-37(47)44-27-31-9-2-1-7-29(31)13-14-30-8-3-4-10-33(30)44/h1-4,7-10,32,34,38H,5-6,11-12,15-28H2,(H,40,46)(H,41,45)(H2,42,43,48)/t32-,34-,38-/m0/s1
• chave InChI: LNHSQAOQVNHUGL-QRBHCBQLSA-N

Descrição

Descrição geral

This azadibenzocyclooctyne-biotin derivative is a versatile biotinylation reagent for labeling azide containing molecules or compounds. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions.This dibenzocyclooctyne will react with azide-functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. Subsequent labeled molecules can be purified using streptavidin or avidin affinity reagents. The PEG spacer makes the overall compound more hydrophilic and allows for greater distance between the reaction site with the azide and the biotin detecting group.

Aplicação

Automate your Biotin tagging with Synple Automated Synthesis Platform (SYNPLE-SC002)

Características e benefícios

More efficient labeling will occur at higher temperatures and with greater excess of biotin reagent. Typical reaction times are up to 4 hours, but longer incubation periods should be used if concentration is low or if low labeling is observed. Product is most efficient when first dissolved in a water miscible organic solvent and then diluted in aqueous reaction buffer when needed. DBCO-PEG4-Biotin is soluble in aqueous buffers up to 0.35 mM.

Outras notas

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Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable