ECACC Laboratory Handbook 4th Edition
Figure 1.Cryopreservation of Cell Lines
The protocol below describes the use of cell freezing methods involving an electric -80 °C freezer for the cryopreservation of cell lines. ECACC routinely use a programmable rate-controlled freezer. This is the most reliable and reproducible way to freeze cells but as the cost of such equipment is beyond the majority of research laboratories the methods below are described in detail. If large numbers of cell cultures are regularly being frozen then a programmable rate-controlled freezer is recommended.
Figure 2.DMSO Bottles
Figure 3.Mr. Frosty Containers
Bright-Line is a trademark of Cambridge Instruments, Inc.
Nalgene is a registered trademark of Thermo Fisher Scientific or its subsidiaries
Preserving cell lines through cryopreservation helps to maintain valuable laboratory resources. If not done properly, freezing cell lines can cause damage to the cells, disrupt your experiments, and negatively influence data collection. This tutorial shows the protocol for freezing cells, tips and tricks to help minimize damage to cells while freezing and the different solutions that are used as cryopreservation media.
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