Fibronectin fragments are obtained from plasma fibronectin by using proteolytic enzymes. These fragments are useful tools for mapping regions within the fibronectin molecule, which are responsible for specific binding to collagen and for other biologically relevant functions.
Click for Fibronectin Mimetics
Explore our 3D cell culture tools
Troubleshoot your cell culture challenges
Click for consistency in cell culture
What is the purity of fibronectin products?
We do not determine the purity of fibronectin solutions. An SDS PAGE gel is run and one major fibronectin band of 120kDa is compared to past lots to ensure consistent quality. All our fibronectin powders are ≥95% pure.
What is the best solvent to dilute fibronectin?
The solvents that are compatible to dilute fibronectins are provided in Table 1.
How long can fibronectin be stored?
Fibronectin products provided as solutions can be stored for a year at 2 - 8 °C provided they are sterile. Diluted, unused fibronectin can be kept at 2 - 8 °C for up to 1 week. Fibronectin-coated plates can be stored at 2 - 8 °C for up to 4 weeks provided they are sealed well to prevent contamination and/or drying up of fibronectin. Do not use the product if discoloration or cracks appear on the surface of the coated material.
What is the optimum concentration of fibronectin for coating?
The coating concentrations of our fibronectins are suggested in Table 1. However, optimizing the concentrations that work for your cells is recommended.
What is the best ECM protein to study stem cell differentiation?
While laminin and fibronectin are widely used ECM proteins, the one that your cell needs should be determined by test experiments. Stem cells express varied integrins on the surface during different stages of differentiation. The choice of ECM protein would depend on the stage of differentiation you are interested in studying.