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Cell Freezing Reagents

Scientist placing cryovial of cells in cryostorage box

Cell lines, primary cells, and genetically-modified cell stocks are among the most valuable and difficult-to-replace resources in the life science, biotechnology, or pharma development laboratory. Techniques and tools for appropriately freezing cells and resuscitating them from cryostorage in liquid nitrogen are among the most critical methods in cell culture. Preventing the formation of intracellular ice crystals and minimizing cell stress is paramount to maintaining cell viability during cryopreservation. We offer an extensive line of sterile-filtered, application-tested cryoprotectants like DMSO as well as ready-to-use cell freezing media with and without DMSO that are designed to maximize cell viability during the freezing and thawing processes.

Hybri-Max sterile filtered DMSO is tested for hybridoma cryopreservation

Ready-to-use Cell Freezing Media

Convenient cell freezing media reagents eliminate the need to titrate DMSO concentrations, and are also available in DMSO-free formulations.

  • CryoStor® cell cryopreservation media is available in 2%, 5%, and 10% concentrations, as well as a DMSO-free formulation, CryoSOFree™.
  • pZerve™ is a cryopreservation solution for serum-containing or serum-free culture that does not contain dimethyl sulfoxide (DMSO), fetal bovine serum or other animal-derived ingredients
  • EmbryoMax® 2X Freezing Medium for ES (embryonic stem) cells, formulated with 20% DMSO & fetal bovine serum
  • HypoThermosol® FRS Preservation Solution enhances and extends storage of cells, tissues, and organs at 2 – 8 °C

DMSO (Dimethyl Sulfoxide) for Cell Cryopreservation

Dimethyl sulfoxide or DMSO is an organic solvent that is used as a cryoprotectant when cells are frozen down for cryostorage. As a component of cell freezing media, DMSO protects cells by preventing the formation of both extracellular and intracellular ice crystals. Choose from DMSO reagents that

  • Meet USP and EP testing specifications
  • Are BioPerformance certified for cell culture
  • Are suitable for hybridoma
  • Are tested for plant cell culture applications

Products

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Product Number
Product Description
Pricing
D2650
Dimethyl sulfoxide, Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
D2438
Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
C2874
CryoStor® cell cryopreservation media, CS10
D4540
Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture
C6164
Cell Freezing Medium-DMSO 1×, sterile-filtered, suitable for cell culture
D1435
Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications
C6295
Cell Freezing Medium-DMSO Serum free 1x, sterile-filtered, suitable for cell culture
C2999
CryoStor® cell cryopreservation media, CS5
H4416
HypoThermosol® FRS Preservation Solution
C9249
CryoSOfree™ DMSO-free Cryopreservation Medium
20-139
Dimethyl Sulfoxide (DMSO)
C3124
CryoStor® cell cryopreservation media, CS2
Z1653
pZerve™, Cryopreservation Solution
C2639
Cell Freezing Medium-Serum-free 1×, sterile-filtered, suitable for cell culture
D2447
Dimethyl Sulfoxide DMSO/Dextran 40, 55% w/v DMSO USP, Ph.Eur. 5% w/v Dextran 40 USP in NaCl
ES-002
EmbryoMax® 2X Freezing Medium for ES Cells, formulated with 20% DMSO & Fetal Bovine Serum, The EmbryoMax 2X Freezing Medium for ES Cells is formulated with 20% DMSO & Fetal Bovine Serum and available in a 10 x 10 mL format.
040-50
Freezing Medium (50 ml)
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Related Product Literature

CryoStor® Cryopreservation Protocol
Cryopreservation efficacy which includes post-thaw recovery, viability, and functionality is of importance to both research and clinical applications. The cumulative stresses that result from the cryopreservation process and suboptimal freeze media result in cell death from necrosis and apoptosis.
Cell Culture Fundamentals: Cryopreservation and Storage of Cell Lines
Overview on liquid nitrogen storage and freezing of cell stocks for cell line cryopreservation purposes. Free ECACC handbook download.

Related Product Categories

Classical Media & Buffers
Cell culture media formulations have been optimized over decades of research in response to diverse cellular physiology. Select media such as DMEM, MEM, RPMI-1640, Ham’s F-10 & F-12 formulations, as well as serum-free solutions such as M199.
Mammalian Cell Lines
Cell lines from human and other mammalian species are essential for modeling disease and biological systems, and are critical tools for protein, antibody, virus, and vaccine production. We offer authenticated, contaminant-free cell lines, many in partnership with ECACC.
Primary Cells
Primary cells are derived directly from living tissue and closely replicate the physiology of biological systems. Our collection includes blood, patient tumor xenografts, and hepatocytes essential for drug screening and ADME/Tox studies.
Stem Cells
Our complete range of ES and iPS cell-qualified products includes stem cell lines and media for differentiation and maturation, and provides stem cell researchers with convenient and cost-effective solutions for the reliable culture of pluripotent stem cells.
Stericup® Vacuum Filters
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Millipore® Sterile Filters
Prepare cell culture media and filter-sterilize aqueous solutions from a few mL to 20 L using industry-leading Millipore® sterile filtration devices. Choose fit-for-application membranes and pore sizes in Steriflip®, Sterivex®, Stericap®, and Steripak® devices.

Related Applications

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Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.
Primary Cell Culture
Primary cells isolated directly from human and animal sources maintain physiological relevance of the tissues from which they are derived, and are used for ADME/Tox screening. Find applications and methods for culturing mammalian primary cells in vitro.
Stem Cell Culture
An overview of different stem cell types, stem cell culture basics and stem cell applications in basic research and clinical research applications, such as tissue regeneration, genetic diseases and cancer.
3D Cell Culture
Applications, principles, and techniques for three-dimensional (3D) cell culture. Includes techniques and descriptions of scaffold and scaffold-free methods matrices for the culture of complex in vitro models including organoids and spheroids.
Plant Tissue Culture
Plant cell and tissue culture in commercial plant production, conservation, and plant research. Meristem culture, virus-free plant propagation, protoplast and haploid plant production.
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Cell Growth & Maintenance
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Highlights

Watch demonstrated procedures for freezing, storage, and thawing of cells, as well as a viability assessment of cells post-thaw.