Poly-L-lysine is a nonspecific attachment factor for cells and is useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-lysine increases the number of positively charged cell binding sites. The following protocol applies to product numbers P4707*, P4832*, P7280, P6407, P7405, P9155, P6282, and P5899.
Optimal conditions must be determined for each cell line and application.
*NOTE: Step 1 is not necessary for poly-lysine solutions (P4707 and P4832).