Poly-L-Lysine Cell Attachment Protocol

George Sitterley

MilliporeSigma

Optimal conditions must be determined for each cell line and application.

  1. Add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine.
  2. Aseptically coat culture surface with 1.0 mL/25 cm2 (only). Rock gently to ensure even coating of the culture surface.
  3. After 5 minutes, remove solution by aspiration and thoroughly rinse surface with sterile tissue culture grade water.
  4. Allow to dry at least 2 hours before introducing cells and medium.


*NOTE: Step 1 is not necessary for poly-lysine solutions (P4707 and P4832).

Materials
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