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  • Podocyte-Specific Deletion of Murine CXADR Does Not Impair Podocyte Development, Function or Stress Response.

Podocyte-Specific Deletion of Murine CXADR Does Not Impair Podocyte Development, Function or Stress Response.

PloS one (2015-06-16)
Christoph Schell, Oliver Kretz, Andreas Bregenzer, Manuel Rogg, Martin Helmstädter, Ulrike Lisewski, Michael Gotthardt, Pierre-Louis Tharaux, Tobias B Huber, Florian Grahammer
ABSTRACT

The coxsackie- and adenovirus receptor (CXADR) is a member of the immunoglobulin protein superfamily, present in various epithelial cells including glomerular epithelial cells. Beside its known function as a virus receptor, it also constitutes an integral part of cell-junctions. Previous studies in the zebrafish pronephros postulated a potential role of CXADR for the terminal differentiation of glomerular podocytes and correct patterning of the elaborated foot process architecture. However, due to early embryonic lethality of constitutive Cxadr knockout mice, mammalian data on kidney epithelial cells have been lacking. Interestingly, Cxadr is robustly expressed during podocyte development and in adulthood in response to glomerular injury. We therefore used a conditional transgenic approach to elucidate the function of Cxadr for podocyte development and stress response. Surprisingly, we could not discern a developmental phenotype in podocyte specific Cxadr knock-out mice. In addition, despite a significant up regulation of CXADR during toxic, genetic and immunologic podocyte injury, we could not detect any impact of Cxadr on these injury models. Thus these data indicate that in contrast to lower vertebrate models, mammalian podocytes have acquired molecular programs to compensate for the loss of Cxadr.

MATERIALS
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Product Description

Sigma-Aldrich
Anti-CXADR antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Carbon nanofibers, pyrolitically stripped, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm
Sigma-Aldrich
Carbon nanofibers, graphitized, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm
Carbon - Vitreous, foil, 50x50mm, thickness 1.0mm, glassy carbon
Carbon - Vitreous, rod, 200mm, diameter 7.0mm, glassy carbon
Carbon - Vitreous, rod, 100mm, diameter 7.0mm, glassy carbon
Carbon - Vitreous, foil, 100x100mm, thickness 1.0mm, glassy carbon
Carbon - Vitreous, rod, 5mm, diameter 3.0mm, glassy carbon
Carbon - Vitreous, foil, 50x50mm, thickness 4.0mm, glassy carbon
Carbon - Vitreous, foam, 150x150mm, thickness 2.5mm, bulk density 0.05g/cm3, porosity 96.5%
Carbon - Vitreous, rod, 200mm, diameter 1.0mm, glassy carbon
Carbon - Vitreous, foil, 10x10mm, thickness 1.0mm, glassy carbon
Carbon - Vitreous, foam, 300x300mm, thickness 20mm, bulk density 0.05g/cm3, porosity 96.5%
Carbon - Vitreous, foil, 25x25mm, thickness 4.0mm, glassy carbon
Carbon - Vitreous, rod, 200mm, diameter 5.0mm, glassy carbon
Carbon - Vitreous, foam, 275x330mm, 0.05g.cmué, porosity 96.5%, 24 pores/cm
Carbon - Vitreous, rod, 100mm, diameter 5.0mm, glassy carbon
Carbon - Vitreous, foil, 100x100mm, thickness 6.0mm, glassy carbon
Carbon - Vitreous, foil, 25x25mm, thickness 0.5mm, glassy carbon
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Carbon - Vitreous, foil, 100x100mm, thickness 2.0mm, glassy carbon
Carbon - Vitreous, foil, 10mm disks, thickness 0.2mm, glassy carbon
Carbon - Vitreous, foam, 150x150mm, thickness 3.2mm, bulk density 0.05g/cm3, porosity 96.5%
Carbon - Vitreous, rod, 100mm, diameter 3.0mm, glassy carbon
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Carbon - Vitreous, rod, 100mm, diameter 1.0mm, glassy carbon